吡啶降解特效菌群的构建与应用
时间:2018-03-21 09:59 来源:毕业论文 作者:毕业论文 点击:次
| 摘要本文研究了具有吡啶降解能力的四株纯菌株Rhizobium sp.NUJST18、Paracoccus thiophilus NJUST11、Paracoccus denitrificans NJUST12和Paracoccus versutus NJUST15的吡啶降解性能，考察了四株菌之间的相互作用，并尝试对此四株菌进行组合培养。研究各个组合条件下的吡啶降解情况。并选出吡啶降解性能较优的组合作为接种物培养好氧颗粒污泥。实验结果表明，NUJST18、NJUST11、NJUST12、NJUST15四株菌均可以以吡啶为唯一碳源和唯一氮源生长，并且吡啶降解的过程伴随着氨氮的释放。抑菌实验结果表明该四株菌之间没有相互抑制作用，可以以之为菌种来源进行菌群构建。组合菌株的降解速率与单一菌株相比，其吡啶降解效率均有所增强；吡啶降解效率随着生物种类的增加而显著提高。以NUJST18，NJUST11，NJUST12和NJUST15四株菌作为混合接种物，菌群可在36h内实现1000mg/L吡啶的完全降解。构建复合菌群的方法在高浓度难降解吡啶废水的处理方面具有良好的应用前景。19898 关键词 菌群 构建 吡啶 生物降解 毕业论文设计说明书（论文）外文摘要 Title The construction and application of community capable of degrading pyridine Abstract This paper studies pyridine degradation performance of the four pure strains with pyridine degradation ability, namely Rhizobium sp. NUJST18, Paracoccus thiophilus NJUST11, Paracoccus denitrificans NJUST12 and Paracoccus versutus NJUST15. The interaction between the four strains was investigated in this study, and combination and cultivation of the four bacteria strain was tried. In addition, the pyridine biodegradation performance of the communities was evaluated. The experimental results showed that the four bacteria strain, namely NUJST18, NJUST11, NJUST12, and NJUST15, could grow with pyridine as the sole carbon and nitrogen sources. Pyridine degradation was accompanied with the release of ammonia nitrogen. Inhibition between the four strains of bacteria was not observed, indicating the feasibility of enhanced pyridine biodegradation by the community composed of the four strains. Compare with single strain, the combined community exhibited higher pyridine degradation efficiency. Pyridine degradation performance increased with the increase of the abundance of the community. The combination of strain NUJST18 and NJUST11 and NJUST12 and NJUST15 exhibited the highest pyridine degradation performance within all combinations. 1000 mg/L of pyridine could be degraded completely within 36 h. Community construction offers bright prospects for the treatment of high-strength and recalcitrant wastewater containing pyridine. Keywords Community Construction Pyridine Biodegradation 目 次 1 引言 1 1.1吡啶的简介 1 1.2含吡啶废水的治理方法 1 1.3 吡啶降解菌群的构建 3 1.4研究课题的提出和内容 5 2 实验材料与方法 7 2.1 实验材料 7 2.2 实验方法 8 2.3 表征手段 10 3 结果与讨论 12 3.1 菌种的鉴定和性能表征 12 3.2 菌之间的相互作用 16 3.3单菌体系与菌群体系的吡啶降解性能比较 17 3.4 复配菌的降解效果 22 3.5 好氧颗粒污泥的性能表征 23 结 论 24 致 谢 25 参考文献26 1 引言 1.1吡啶的简介 吡啶及其衍生物在是一类典型的难降解含氮杂环化合物,普遍存在于地面和地下土壤中，它们在环境中具有持久性的特点，可长期存在于环境中而对环境产生不良影响[1]。由于吡啶及其衍生物具有水溶性较强的特点，极易转移到地下水中而污染地下水。而且，它们的致畸变特性和毒性对健康具有潜在的危害。随着吡啶及其衍生物在农药、化工、医学医药等领域内的不断发展和应用,所带来的环境污染和健康问题日益引起人们的关注[2]。 (责任编辑：qin) |