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摘  要本研究报道野生型矮牵牛对甲醛胁迫的分子响应分析：用15 mg/Ｌ的甲醛溶液处理野生型矮牵牛24 h，矮牵牛经甲醛胁迫后，测序结果显示差异性基因有2800个，上调基因有1300个，下调基因有1500个。按通路名称划分，与不同氨基酸代谢通路相关的有11个，占24%；与光合作用相关的代谢通路有6个，占13%；与防御与解毒的通路有3个，占6%；信号转导通路2个,占4%；其它的就是与生物合成、辅酶因子及萜类合成等有关，占据极少的比例。植株体内谷胱甘肽转移酶、细胞色素P450、咖啡酰辅酶A等基因均有上调表达趋势，同样矮牵牛受到甲醛胁迫后，其植物体内叶绿体的叶绿素a-b结合蛋白、水通道蛋白、叶绿体的光合系统蛋白等基因均有下调表达趋势，根据这些测序所得的结果，与qRT-PCR验证试验，结果是一致的。88329

Abstract This study analyzed molecular response of wild type petunia on formaldehyde stress。 Wild type petunia was cultivated in the culture bottle which is filled with 15 g/L formaldehyde solution in artificial climate chamber processing after 24 h。 After formaldehyde stress, sequencing was conducted and the results showed that activities of 1300 genes were up-regulated and activities of 1500 genes down-regulated among 2800 genes。 Based on the KEGG pathway classification, 11 pathways associated with amino acid metabolism and were 24% of the total; 6 pathways associated with photosynthesis and were 13% of the total; 3 pathways associated with defense and detoxification and were 6% of the total; 2 pathways associated with signal transduction and were 4% of the total。 And other pathways associated with biosynthesis, coenzyme factor and terpenoids synthesis, accounted for a little percent。 Petunia under formaldehyde stress, glutathione S-transferas, cytochrome P450 CYP, caffeoyl-CoA and other enzymes genes with functions of defense and detoxification of them have been up-regulated tendency。 At the same time, chlorophyll a-b binding protein, aquaporin protein and chloroplast protein of photosynthetic system have been down-regulated tendency。 The results indicate that formaldehyde destroyed the plant photosynthetic system which changed the metabolic pathways。

毕业论文关键词：矮牵牛； 甲醛胁迫； 基因表达；分子机理

Keyword: Petunia hybrida； formaldehyde stress； gene expression；molecular mechanism

目    录

引言 4

1 材料与方法 4

1。1 实验材料 4

1。2 方法 5

1。2。3 提取RNA 5

1。2。4 构建cDNA文库 6

1。2。5 测序数据源-于,优W尔Y论L文.网wwW.youeRw.com 原文+QQ75201,8766的处理 7

1。2。6 甲醛处理前后差异表达分析 7

1。2。7 EST功能注释及分析 8

1。2。8 验证差异性基因 8

1。2。9荧光定量PCR验证结果的统计学分析 14

2。 结果与分析 14

2。1 测序的数据质量统计结果及Reads质量评估 14

2。2 差异表达分析 16

2。2。2 EST的差异表达分析 17

2。3 EST功能注释及分析的结果 18

2。4 验证差异性基因的结果 23

3。讨论 30