褪黑素对萝卜芽苗菜花青苷合成的影响
时间:2018-07-11 21:02 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：以萝卜（Raphanus sativus L.）芽苗菜为试验对象，在LED白色光源下外源施加褪黑素处理萝卜芽苗菜，以去离子水为对照，研究褪黑素对萝卜芽苗菜花青苷合成的影响。从结果可以看出，与对照相比，褪黑素处理能够显著提高‘杨花萝卜’芽苗菜的下胚轴中花青苷含量和DPPH清除能力，对‘潍县萝卜’芽苗菜花青苷含量和DPPH清除能力没有影响。‘杨花萝卜’芽苗菜下胚轴的PAL和CHI活性均高于‘潍县萝卜’芽苗菜下胚轴的酶活性。此外在‘杨花萝卜’芽苗菜中，随着培养时间的增加，PAL和CHI活性逐渐增加，并且照光3 h后褪黑素处理下PAL和CHI的酶活性均大于对照。此外，与对照相比，褪黑素处理下‘杨花萝卜’芽苗菜中调控花青苷合成的转录因子PAP1和PAP2和关键结构基因CHI，F3H，F3’H，DFR，ANS等的表达量都显著上调。25626 毕业论文关键词：花青苷；萝卜芽苗菜；褪黑素；抗氧化能力 The effects of melatonin on biosynthesis of anthocyanin in radish sprouts Abstract: In this research, we use radish(Raphanus sativus L.) sprouts as experimental material. We treat it under white light with melatonin added and take the group growing in dH2O without melatonin as control, to research into the effect of melatonin on biosynthesis of anthocyanin in radish sprouts. The result shows that, compared with control group, melatonin significantly improved the concentration of anthocyanin in hypocotyl of Yanghua and its ability of cleanning DPPH. However, melatonin has no sensible effect on Weixian radish spouts. At the same time, the activity of PAL and CHI in hypocotyl of Yanghua both are higher than that of Weixian. Besides, as the time of cultivation goes, after 3 h of illumination, PAL and CHI in Yanghua radish sprouts gets more active and those with melatonin have higher PAL and CHI activity than control group. In addition, compared with control group, the expression of transcription factors PAP1 and PAP2 and key structural genes such as CHI, F3H, F3’H, DFR, ANS in Yanghua radish spouts treated with melatonin are all upregulated. Key words: Anthocyanin; radish sprouts; melatonin; antioxidant 目 录 摘要 2 关键词 2 Abstract. 2 Key words 2 引言 2 1 材料与方法 5 1.1 试验材料与试验方法 5 1.2 形态生长指标的测定 5 1.3 花青苷含量及DPPH清除能力的测定 5 1.4 PAL、CHI酶活性测定 5 1.5 基因表达量的检测 6 1.6 数据处理及统计方法 7 2 结果与分析 7 2.1 褪黑素浓度的筛选 7 2.2 褪黑素对萝卜芽苗菜生长的影响 8 2.3 褪黑素对萝卜芽苗菜花青苷含量及DPPH清除能力的影响 8 2.4 褪黑素对萝卜芽苗菜PAL，CHI酶活性的影响 10 2.5 褪黑素对萝卜芽苗菜花青苷合成关键基因表达量的影响 11 3 讨论 12 致谢 13 参考文献： 13 褪黑素对萝卜芽苗菜花青苷合成的影响 引言 萝卜芽苗菜风独特，是一种绿化型芽苗菜，富含大量对人体有益的营养，如文生素C、硫代葡萄糖苷、酚类物质等，在中国及东南亚国家深受欢迎[1-4]。花青苷是一种酚类化合物，是构成植物颜色的主要水溶性色素之一[5]。许多果蔬中都富含花青苷，如在黑莓、红葡萄、蓝莓果实中花青苷的含量分别达到 3.26、7.5和4.95 mg/g[6]。 (责任编辑：qin) |