DL-蛋氨酸对IUGR哺乳仔猪肝脏糖代谢
时间:2018-07-12 11:53 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：本试验旨在研究DL-蛋氨酸对宫内发育迟缓（intrauterine growth retardation, IUGR）哺乳仔猪肝脏糖代谢的影响。试验在新生仔猪中选择日龄相同的5头正常出生重（normal birth weight, NBW）仔猪和10头IUGR仔猪，分为3个处理组（n = 5/组）：NBW-CON组（正常仔猪+基础人工乳），IUGR-CON组（IUGR仔猪+基础人工乳）和IUGR-MET组（IUGR仔猪+基础人工乳+0.12% DL-蛋氨酸），21日龄时屠宰取样。结果显示：（1）与NBW-CON组相比，IUGR-CON组磷酸烯醇式丙酮酸羧激酶和葡萄糖-6-磷酸酶活力明显升高（P < 0.05）；（2）与NBW-CON组相比，IUGR-CON组糖原合成酶明显降低（P < 0.05）；（3）与NBW-CON组相比，IUGR-CON组磷酸烯醇式丙酮酸羧激酶1和葡萄糖-6-磷酸酶mRNA表达明显升高（P < 0.05），且糖原合成酶mRNA表达显著降低（P < 0.05）；与IUGR-CON组相比，IUGR-MET组磷酸烯醇式丙酮酸羧激酶和葡萄糖-6-磷酸酶有下降的趋势（0.05 ≤ P < 0.1），但显著降低了二者mRNA表达水平（P < 0.05）。试验结果表明，添加DL-蛋氨酸可在一定程度缓解IUGR引起的肝脏糖异生酶活水平升高，显著降低IUGR哺乳仔猪肝脏糖异生酶mRNA表达水平。25686 毕业论文关键词：DL-蛋氨酸；宫内发育迟缓；肝脏；糖代谢； Effects of DL-Methionine on hepatic glucose metabolism in IUGR suckling piglets Abstract：This experiment was to study DL-methionine on hepatic glucose metabolism of intrauterine growth retardation (IUGR) suckling piglets. 5 normal birth weight (NBW) piglets and 10 IUGR piglets were pided into three treatment group (n = 5/group): NBW-CON group (NBW piglets fed based artificial milk), IUGR-CON group (IUGR piglets fed based artificial milk) and IUGR-MET group (IUGR piglets fed based artificial milk + 0.12% DL methionine). The piglets were slaughtered for samples at 21 d of age. Results showed that: (1) Compared with NBW-CON group, the phosphoenolpyruvate carboxykinase and glucose-6-phosphatase activities were significantly higher than IUGR-CON group (P < 0.05); (2) Compared with NBW-CON group, the glycogen synthetase activity of IUGR-CON group was mildly decreased (0.05 ≤ P < 0.1); (3) Compared with NBW-CON group, the mRNA levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphataseof IUGR-CON group were significantly increased (P < 0.05), and glycogen synthetase mRNA levels were significantly reduced (P < 0.05); Compared with IUGR-CON group, the mRNA levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase of IUGR-MET group were significantly increased (P < 0.05), and the activities of these two enzymes were mildly reduced (0.05 ≤ P < 0.1).These results show that DL- methionine may have ability to improve hepatic gluconeogenesis metabolism in IUGR suckling piglets. Keywords：DL-methionine; IUGR; liver; glucose; metabolism; 目 录 摘要 1 关键词 1 Abstract 1 Keywords 1 引言 1 1 材料与方法 2 1.1 试验设计 2 1.2 样品采集 2 1.3 测定指标及方法 2 1.3.1 肝脏糖代谢评估 2 1.3.2 mRNA表达量 3 1.4 数据统计与分析 3 2 结果与分析 4 2.2 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖原代谢的影响 4 2.3 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖酵解酶活力的影响 5 2.4 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏mRNA水平的影响 5 3 讨论 6 3.1 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖异生酶活力及mRNA水平的影响 6 3.2 DL-蛋氨酸对21日龄IUGR哺乳仔猪肝脏糖原代谢的影响 7 (责任编辑：qin) |