不同浓度Cd2+对HNU-1和HNU-1(epsA-O)产生物膜的影响_毕业论文

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不同浓度Cd2+对HNU-1和HNU-1(epsA-O)产生物膜的影响

摘要: 目前,全球的重金属污染,特别是镉离子污染日益加重,水资源土壤资源等均被严重污染。镉污染既影响农作物产量,减少经济收益,也会通过食物链进入人体,严重危害人体生命健康。因此寻求简便有效的镉离子环境修复方法显得尤为重要。在前期研究中,我们发现菌株HNU-1能够通过产生物膜固定环境中镉离子。本研究旨在探索不同浓度镉离子对菌株产生物膜情况的影响。首先构建了菌株HNU-1的胞外多糖缺失突变体HNU-1(epsA-O)做对照,并且检测了不同浓度镉离子对野生型菌株HNU-1和突变体菌株HNU-1(epsA-O)生物膜产生情况的影响。发现野生型菌株HNU-1能在低浓度镉离子条件下产生物膜,但在高浓度镉离子条件下生长受到抑制;而突变体菌株HNU-1(epsA-O)在低浓度的镉离子条件下生长即受到抑制。93329

毕业论文关键词:枯草芽孢杆菌,生物膜,镉离子

Abstact: At present, the pollution of heavy metals in the world, especially the pollution of cadmium ion is increasing day by day。 Cadmium pollution not only affects crop yield, reduce economic returns, but also through the food chain into the human body, serious harm to human life and health。 Therefore, it is very important to find a simple and effective method of environmental remediation of cadmium ion。 In previous studies, we found that strain HNU-1 was able to immobilize the cadmium ion in the environment through the membrane。 The purpose of this study was to explore the effects of different concentrations of cadmium on biofilm formation。 The strain HNU-1 was constructed of extracellular polysaccharide mutant HNU-1 (epsA-O) as control, and the detection of different concentrations of cadmium ions on HNU-1 and the mutant strain HNU-1 of wild type strain (epsA-O) biofilms produced by。 Found that the wild type strain HNU-1 in biofilm producing low concentration cadmium ion conditions, but in high concentrations of cadmium ions inhibited the growth conditions; and the mutant strain HNU-1 (epsA-O) on the growth conditions of low concentration of cadmium ion is suppressed。源F于K优B尔C论V文N网WwW。youeRw。com 原文+QQ752^018766

Keywords: Bacillus subtilis,biofilm,cadmium ion

目 录

目 录 3

1  前言 3

材料与方法 5

2。1  菌株活化培养 5

2。2 突变体的构建 5

2。3  培养基及培养液的配制 5

2。4  HNU-1和HNU-1(epsA-O)在不同Cd2+浓度的培养基中生长情况检测 5

3  结果与分析 6

4  结论 7

参考文献 7

致谢 9

1  前言来自优O尔P论R文T网WWw。YoueRw。com 加QQ7520`18766

由于工业化进程的发展、水资源和土地资源的不合理利用以及废物的不合理处理,重金属污染已成为全球越来越严重的环境问题[1,2]。这些重金属元素中镉已经成为威胁农业生产的罪魁祸首。镉是全球性危险化学物质中位于首位的有毒重金属[3]。全球每年释放到环境中的镉高达3。9 ×104 t[5]。而在环洪泽湖地区造成污染的几种重金属当中,镉的污染程度也要高于其他几种重金属[6]。并且镉极易污染土壤,由于农作物的吸收作用再通过农作物进入人体,危害人体生命健康[4]。,因此,研究重金属镉的环境修复方法是极其必要的。 (责任编辑:qin)