日粮氮源与乳酸链球菌素对湖羊瘤胃发酵血液生化及氮平衡的影响
时间:2018-08-01 14:25 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：本试验旨在通过饲养试验研究日粮添加乳酸链球菌素（Nisin）对两种不同氮源条件下湖羊瘤胃发酵、血液生化及氮平衡的影响。采用2×2因子设计，两因子分别为氮源（豆粕、DDGS）和乳酸链球菌素（Nisin，0、30.5mg/kg日粮），配置四种日粮。选取32只23kg左右的断奶公湖羊，按照随机区组设计原则，根据体重分为4个处理，分别饲相应日粮。试验期共9周，试验期期内测定采食量，并采集血液、瘤胃液以及粪样和尿样。结果显示：与豆粕氮源处理相比，DDGS氮源处理湖羊瘤胃乙酸、丁酸、及支链挥发酸浓度显著降低（P < 0.05），总挥发性支链脂肪酸有降低的趋势（P < 0.05）；与豆粕组相比，DDGS作为氮源显著降低瘤胃氨氮浓度和血液尿素氮浓度及尿氮排放量（P < 0.05），显著增加粪氮排放（P < 0.05），对氮沉积无显著影响（P > 0.05）。而在日粮中添加乳酸链球菌素对湖羊瘤胃发酵参数、血液生化指标和氮平衡指标均无显著影响（P > 0.05）。结果表明：用DDGS替代豆粕作为日粮氮源可降低湖羊瘤胃氨氮、血液尿氮浓度，进而减少尿氮排放。26569 毕业论文关键词：乳酸链球菌素；湖羊；瘤胃发酵；血液生化；氮平衡 Effects of Nitrogen Source and Nisin on Rumen Fermentation, Blood Metabolites and Nitrogen Balance of Hu sheep Abstract: This test is to add by feeding experiment study nisin of the two sheep rumen fermentation under the conditions of different nitrogen source, blood biochemistry and the influence of nitrogen metabolism. The two factors were designed with 2 by 2 factors, and the two factors were the nitrogen source (soybean meal, DDGS) and lactate streptococcus (0, 30.5 mg/kg), and the four kinds of daily grain. Select 32 only 20 kg of weaning male sheep, according to randomized block design and with the principle of equal size, according to the weight is pided into four, respectively fed diet accordingly, each processing 8 sheep, single bar feeding, free drinking water. The experiment was based on the 46:54 (DM base) total mixed daily grain (TMR) as the base day food, 10 weeks in the trial period, and the first two weeks were the adaptation period. The group C based diet fed soybean meal as nitrogen source, group D fed soybean meal as nitrogen source to add 30.5 mg/kg Nisin, treatment group A and B in the add DDGS as nitrogen source based diet added respectively 0 mg/kgNisin and 30.5 mg/kgNisin. The amount of consumption was measured in the eighth week of the trial period. In the 8th week, the samples were collected for 3 days and used for the determination of nitrogen metabolism. The gastric juice was collected from the gastric juice of all the lakes, and was used for the analysis of fermentation parameters after feeding on the 6th week of the experimental period and the following morning of the 8th week. 6 and 8 weeks of the trial period, in the morning between 3 h after feeding on sheep all trials in jugular vein quantitative blood 10 ml, 15 minutes 3000 RPM centrifugal separation of serum, serum on - 20 ℃ to save to the analysis, then you can get blood analysis data. The result shows: the soybean meal protein rumen degradation rate is high, rumen DDGS protein degradation rate is low, and streptococcus element to sheep rumen fermentation had no significant influence (P > 0.05), and has a significant effect on sheep blood biochemical (P < 0.01), blood glucose, albumin, total protein, etc all have certain difference. In the case of nitrogen metabolism, the results showed that the rate of degradation of the soybean meal was high, the degradation rate of the DDGS was low, and the substitution of DDGS for soybean meal to reduce the nitrogen discharge. The nisin can inhibit the removal of amino acids from the rumen and improve the efficiency of nitrogen. Key words: Nisin; Hu sheep; Rumen fermentation; Blood biochemical; Nitrogen balance 目录 (责任编辑：qin) |