乙烯和生长素在番茄果实形成过程中互作的探究
时间:2018-10-29 15:25 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：EIL（Ethylene-insensitive 3-like）是乙烯信号通路中重要的转录因子。本实验发EIL共抑制转基因番茄（EIL-CS line）出现单性结实现象，经测定发现其单性结实果实的内源生长素浓度明显高于EIL过表达和野生型对照植株，说明抑制EIL的转录能够提高开花后子房中生长素含量，从而引起单性结实。为了进一步探究乙烯与生长素在果实形成过程中的互作关系，本实验通过研究EIL-OX、pEIL-GUS和DR5-GUS等转基因番茄，分析了EIL蛋白和生长素在花蕾中的表达和合成规律，结果显示，开花前期，EIL超表达（EIL-OX）番茄中生长素生物合成高于野生型对照，开花后生长素的合成无明显差异。生长素在乙烯信号关键因子EIL的影响下，在开花前后表现出不同的合成规律，说明开花前后EIL与生长素的互作机制存在不同。29786 毕业论文关键词：乙烯 ；生长素；番茄；果实发育 Study on the Interaction of Ethylene and Auxin in Tomato Fruit Development Abstract：EIL (Ethylene-insensitive3-like) is an important transcription factor in the ethylene signaling pathway. In this study, Parthenocarpic fruit set was observed in EIL co-suppressed transgenic tomato (EIL-CS line). The concentration of endogenous auxin in the fruit of EIL- CS was significantly higher than that of EIL overexpression and wild type control plants, which indicated that inhibiting the transcription of EIL could increase the content of auxin in the ovary after flowering. In order to further understand the interaction between ethylene and auxin in the process of fruit formation, the expression pattern of EIL and synthesis of auxin in buds were studied via EIL-OX, pEIL-GUS and DR5-GUS transgenic tomatoes. The results showed that the auxin biosynthesis of EIL-OX was higher than that of wild type control, but there was no significant difference in the synthesis of auxin after flowering. Under the influence of EIL, the auxin showed different synthetic characteristics before and after flowering, indicating that the mechanism of interaction between EIL and auxin was different before and after flowering. Key words: ethylene；auxin；tomato；fruit development 目录 摘要 1 关键词 1 Abstract 1 Key words 1 引言： 1 1 材料与方法 2 1．1 试验材料 2 1．2 EIL1-OX/DR5-GUS转基因番茄的培养 2 1．3 转基因植株表型观察及取样 2 1．4 GUS染色液的配制及染色鉴定 2 1．4．1 GUS染色液的配制 2 1．4．2 GUS染色鉴定 2 1．5 EIL1浓度的测定 3 1．5．1 样品RNA的提取 3 1．5．2 cDNA合成 3 1．5．3 PCR 3 1．5．4 数据处理 3 1．6 生长素浓度测定 3 2 结果与分析 3 2．1 EIL1在番茄果实中的表达模式 3 2．2 转基因番茄表型观察 4 2．2．1 EIL对番茄植株发育的影响 4 2．2．2 EIL对番茄果实发育的影响 5 2．3 果实内源生长素浓度测定 5 2．4 通过GUS染色检测果实形成前期（开花阶段）EIL1调控生长素合成的规律 5 2．4．1 EIL1超表达对番茄果实子房发育的影响 6 2．4．2 EIL1超表达对生长素的影响 6 3 讨论 8 3．1 EIL1与单性结实 8 (责任编辑：qin) |