姜黄素对IUGR鼠肌肉抗氧化机制的研究
时间:2018-11-23 09:45 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：本试验旨在研究日粮添加姜黄素对宫内发育迟缓（intrauterine growth retardation, IUGR）鼠肌肉抗氧化机制的影响。试验采用2×2因子设计，12只正常仔鼠和12只IUGR仔鼠21日龄断奶后随机分成四个处理组（每组6只）：NBW组为正常仔鼠，饲喂基础日粮；NC组为正常仔鼠，饲喂基础日粮+400 mg/kg姜黄素；IUGR组为IUGR仔鼠，饲喂基础日粮；IC组为IUGR仔鼠，饲喂基础日粮+400 mg/kg姜黄素。断奶适应期1周，正式试验期4周。结果显示：（1）与NBW组相比，IUGR组大鼠肌肉丙二醛（malondialdehyde, MDA）含量显著升高（P < 0.05），谷胱甘肽过氧化物酶（glutathione peroxidase, GSH-PX）活力显著降低（P < 0.05）。日粮添加姜黄素可显著降低IUGR大鼠肌肉MDA含量（P < 0.05），显著升高总超氧化物歧化酶（totalsuperoxide dismutase, T-SOD）活力（P < 0.05）；（2）与NBW组相比，IUGR组大鼠肌肉核因子NF-E2相关因子2（nuclear factor erythroid-2 related factor 2, Nrf2）、超氧化物歧化酶1（superoxide dismutase, SOD1）基因mRNA的表达水平均显著降低（P < 0.05）。日粮添加姜黄素可显著提高IUGR组大鼠肌肉Nrf2、SOD1基因mRNA的表达水平（P < 0.05）。试验结果表明，日粮添加400 mg/kg姜黄素可有效缓解IUGR引起的大鼠肌肉氧化损伤。30508 毕业论文关键词：姜黄素；IUGR；抗氧化 Effects of curcumin on anti-oxidation in the muscle of IUGR rats Abstract: The present study was designed to evaluate the effects of dietary supplementation of curcumin on anti-oxidation in the muscle of IUGR (intrauterine growth retardation) rats. Twelve normal and twelve IUGR newborn rats were randomly assigned to the following four experimental groups (n = 6/group): NBW group (normal rats, fed with basic diets), NC group (normal rats, fed with basic diets + 400 mg/kg curcumin), IC group (IUGR rats, fed with basic diets) and IC group (IUGR rats, fed with basic diets + 400 mg/kg curcumin). Rats were fed from 4 to 8 weeks of age，including a one-week adaptive phase for weaning. The results indicated that: (1) compared with NBW group, IUGR rats had a higher content of MDA (malondialdehyde) in the muscle (P < 0.05). Furthermore, the activities of GSH-PX(glutathione peroxidase) was significantly decreased in muscle of the IUGR rats (P < 0.05) as compared with that of NBW group. Curcumin significantly reduced the content of MDA (P < 0.05), whereas increased the activities of T-SOD (totalsuperoxide dismutase) in the muscle of IUGR rats (P < 0.05); (2)compared with NBW group, the mRNA expression of Nrf2 (nuclear factor erythroid-2 related factor 2) and SOD1 in the muscle of IUGR rats were significantly down-regulated (P < 0.05). However, curcumin up-regulated these mRNA expressions (P < 0.05). In conclusion, 400 mg/kg curcumin can effectively alleviate the damage by IUGR and had beneficial effects on the oxidative damage in the muscle of IUGR rats. Key words: Curcumin; IUGR; anti-oxidation 目 录 摘要 1 Abstract 1 key words 2 引言 2 1 材料与方法 2 1.1 试验设计 3 1.2 饲养管理 3 1.3 样品采集 3 1.4 测定指标及方法 3 1.4.1 肌肉组织抗氧化及氧化相关指标测定 3 1.4.2 肌肉组织中目的基因mRNA表达量测定 3 1.5 数据统计与分析 4 2 结果与分析 4 2.1 日粮添加姜黄素对正常和IUGR大鼠肌肉抗氧化系统的影响 4 2.2 日粮添加姜黄素对正常和IUGR大鼠肌肉抗氧化相关基因表达的影响 5 3 讨论 6 (责任编辑：qin) |