鸭PRKAB1基因多态性研究
时间:2019-04-13 14:31 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：本试验以北京鸭为材料，根据鸭PRKAB1基因序列设计引物，采用了PCR-SSCP技术和DNA测序技术，检测了北京鸭共122个个体PRKAB1基因单核苷酸多态性，分析了北京鸭的该基因的遗传结构多样性与其饲料转化率的相关性，从而检验这个基因的多态对北京鸭饲料转化率的遗传效应，希望能够发现对饲料转化率有显著效应的遗传标记，以便提高肉鸭的选育的效率，并可以为分子标记数据库的建立和种质资源保护等提供遗传学依据[1]。本研究共检测了PRKAB1基因的8个基因座，其中仅PRKAB1-1基因座表现有SNP位点，而其余的七个基因座均呈单态。根据位点多态性可分成AA、AB、BB三种基因型，其中A等位基因的频率为0.6148，B为0.3852。经过卡方合适性检验，发现各基因型之间不符合Hardy - Weinberg平衡定律。通过本试验可以发现在北京鸭群体中，PRKAB1基因座不同基因型个体与饲料转化率存在相关性(P<0.05)，且AB基因型个体料重比水平显著高于BB基因型个体（P<0.05）。34459 毕业论文关键词：北京鸭、SNP、PRKAB1基因、多态性、饲料转化率 The Polymorphism of PRKAB1 Gene in Duck Abstract: In this study Peking duck were used as the test material and primers were designed according to the sequence of PRKAB1 gene.We used the PCR-SSCP technique and DNA sequencing technology to detect the single nucleotide polymorphisms of Peking duck’s PRKAB1 gene in 122 inpidual . The Peking Duck genetic structure of the gene and its correlation persity was analyzed, so that to examine the association between polymorphism of this gene and effects of genotypes of candidate genes on feed conversion rate of Peking duck, hoping to find the feed conversion rate has a significant effect of genetic markers, in order to improve feed conversion rate of breeding ducks, and to provide genetic information for foundation of molecular marker database, protection and usage of breed resource of Peking duck[1]. This study examined eight loci PRKAB1 gene locus which only PRKAB1-1 manifestations of the SNP, while the remaining seven loci showed a single state. According polymorphism can be pided into AA, AB, BB three genotypes, and allele frequency of A is 0.6148, B is 0.3852. After chi-square proper test ,we found the genotypes does not meet Hardy - Weinberg equilibrium. Through this experiment ,we can found that in Peking duck , the duck of different genotypes in PRKAB1 loci were related to feed conversion rate (P <0.05) and , and AB genotypes feed weight ratio was significantly higher than BB genotype (P < 0.05). Keywords: Peking duck, SNP, PRKAB1 gene, polymorphism, feed conversion rate 目录 前言 1 1材料与方法 2 1.1 试验材料 2 1.1.1 试验动物 2 1.1.2 试验主要试剂 2 1.1.3 溶液与缓冲液 2 1.1.4 仪器设备 3 1.2 试验方法 3 1.2.1 血样的采集 3 1.2.2体尺指标的获得 3 1.2.3鸭全血基因组DNA的提取[14] 3 1.2.4 DNA质量的检测、纯化及浓度计算 3 1.2.5 PCR反应所用引物的设计 3 1.2.6 PCR-SSCP DNA标记分析 4 1.3统计分析方法 5 1.3.1 基因频率和基因型频率的计算 5 1.3.2 基因频率和基因型频率的差异显著性检验 5 1.3.3 不同基因型的个体之间产肉性状的相关分析及显著性检验 6 2结果与分析 6 2.1 北京鸭基因组DNA样品的检测 6 2.2 北京鸭PRKAB1基因座结果与分析 6 2.2.1 PRKAB1基因座 PCR扩增结果及SSCP 分析 6 (责任编辑：qin) |