吡虫啉在玉米中的残留检测方法研究
时间:2019-05-17 19:30 来源:毕业论文 作者:毕业论文 点击:次
| 摘要：本文建立了高效液相色谱检测土壤和玉米中吡虫啉残留的方法。土壤、玉米籽粒和植株样品分别采用乙腈、二氯甲烷提取, SPE固相萃取柱净化, 通过高效液相色谱紫外检测器进行检测。在0.02~10 mg/L范围内,吡虫啉浓度与色谱峰面积呈良好线性关系, 相关系数为1。其最小检出量为0.06ng，最低检出浓度为0.01 mg/kg。在添加浓度为0.02 ~ 1 mg/kg范围内，玉米籽粒中平均回收率为85.5 ~ 98.9%；玉米植株中平均回收率为 86.5 ~ 93.1%；添加浓度为 0.01 ~ 1 mg/kg范围内，土壤中平均回收率为 90.7 ~ 94.8%。该方法的准确度和精密度符合农药残留分析要求，操作简便快速, 节约溶剂, 可为吡虫啉在玉米中的残留分析提供技术依据。35413 毕业论文关键词：吡虫啉；玉米；农药残留；高效液相色谱 Study on the residue detection of Imidacloprid in Maize Abstract: An efficient method was developed for the quantification of imidacloprid residues in soil and maize by high performance liquid chromatography (HPLC). Soil and plant samples were extracted with acetonitrile, dichloromethane, SPE solid phase extraction column and detected by high performance liquid chromatography (UV) detector. In the range of 0.02~10 mg/L, the concentration of imidacloprid was linear with the peak area of the chromatogram, and the correlation coefficient was 1. The limit of detection(LOD) was 0.06 ng and the limit of quantitation(LOQ) was 0.01 mg/kg. The average recoveries of maize were range from 85.5 to 98.9% at spiked of 0.02~1 mg/kg, and the recoveries of maize plant were 86.5~93.1%. For the soil sample, the recoveries were in the range of 90.7~94.8% at the spiked of 0.01~1 mg/kg. The accuracy and precision of this method are in accordance with the requirements of pesticide residue analysis. The method is simple, rapid to operate and lower consumption of solvents, which can provide technical basis for the analysis of imidacloprid in maize and soil. Key words: Imidacloprid; High performance liquid chromatography (HPLC). Maize; Residue analysis 目 录 摘要 1 关键词 1 Abstract 1 Key words 1 1 选题背景 1 1.1 农药残留 1 1.2 吡虫啉的残留分析方法研究进展 2 2 材料与方法 3 2.1仪器设备 3 2.2试剂 3 2.3色谱条件 3 2.4 吡虫啉标准溶液配制 3 2.5 建立吡虫啉标准曲线 3 2.6样品前处理 3 2.6.1 样品提取 3 2.6.2 样品净化 3 2.7 添加回收实验 4 3 结果与分析 4 3.1 建立标准曲线 4 3.2 添加回收率与相对标准偏差 5 4 讨论与结论 5 4.1 仪器参数选择 5 4.2 流动相对色谱分离的影响 6 4.3 样品先处理优化 6 4.3.1 提取溶剂的选择 6 4.3.2 净化方法的选择 7 4.4结论 7 致谢 7 参考文献 7 吡虫啉在玉米中的残留检测方法研究 1 选题背景 1.1 农药残留 农药在人类的生产生活中有着重要的意义，特别是在减少劳动力需求，防治植物病虫害，培育新的作物品种等方面起到了关键的作用，为人类社会的经济效益和社会效益提供巨大发展动力。初期的农药以快速杀死和消灭有害生物为根本，产品毒性相对较高，加之人们环保意识相对薄弱，对农药的相关理论知识不了解造成使用不合理，导致了农药残留超标等问题的出现。 (责任编辑：qin) |