可降解木质素的盐单胞菌菌株YC的分离与鉴定
时间:2020-06-13 11:49 来源:毕业论文 作者:毕业论文 点击:次
| 摘要 本实验分离鉴定出的 YC 菌株，为革兰氏阴性（G-） 、 菌落呈白色，较小；细胞杆状，周生鞭毛，35℃是菌株YC 的最适生长温度，最适氯化钠浓度为 22.8 g/L，最适PH值为 7.5，经16S rDNA序列的测序与系统发育分析表明 YC 菌株与Halomonas titanicae BH1T (AOPO01000038)聚为一支，置信度为 98%。鉴定为盐单胞菌属（Halomonas sp.nov.）的Halomonas titanicae。本实验中用木质素为唯一碳源筛选出的 YC 菌株具有降解木质素的能力，关于盐单胞菌对木质素的降解在国内外都几乎未见报道。所以关于 YC 菌株降解木质素的途径、机制、条件、降解木质素的效率以及如何投入大规模的含木质素的工业废水的处理，在应用于实际时会否引起生物污染等问题都有待进一步深入的研究。 该论文有图 5幅，表4个，参考文献 10篇 50768 毕业论文关键词: Halomonas titanicae 木质素 分离与鉴定 16SrRNA 生理生化特性 Isolation and identification of one kind of biodegradable lignin salts of Aeromonas strains YC Abstract This experiment isolated and identified strains of YC, Gram-negative (G-), white colonies were smaller; rod-shaped cells, Zhou flagella, 35 ℃ is the optimum growth temperature of the strain YC optimum sodium chloride at a concentration of 22.8 g / L, the optimum PH value of 7.5 by sequencing 16S rDNA sequence and system development analysis showed YC strains Halomonas titanicae BH1T (AOPO01000038) clade, confidence level of 98%. Salt identified as Xanthomonas (Halomonas sp.nov.) Of Halomonas titanicae. The experiment using lignin as the sole carbon souYCe screened YC strains with the ability to degrade lignin, on Halomonas lignin degradation at home and abroad have almost been reported. So way on YC strain degrading lignin, mechanisms, conditions, lignin degradation efficiency and how to deal with large-scale investment in lignin-containing industrial waste water, when in reality it will be applied to biological pollution caused by other issues have yet to be further ReseaYCh. Key words: Halomonas titanicae Lignin Isolation and identification 16SrRNA Physiological and biochemical characteristics 目录 摘要 . I Abstract II 目录 . III 1引言 . 4 2材料与方法 . 4 2.1实验材料 .. 4 2.2菌的分离培养 . 5 2.3 菌株的富集与纯化筛选.. 5 2.4 YC菌株的鉴定 5 2.5 YC菌株的生理生化特性 . 7 3结果与分析 . 8 3.1木质素降解菌株 YC的分离纯化与其形态特征 . 8 3.2木质素降解菌 YC菌株的系统发育分析 . 8 3.3 YC菌株的生理生化特性 11 4.结论 .. 15 参考文献 16
致谢 .. 17 |