碱胁迫对花生叶片渗透调节物质的影响研究
时间:2020-06-14 20:03 来源:毕业论文 作者:毕业论文 点击:次
| 本实验的实验材料是红花生种子， 探究在常温条件下花生叶片中渗透调节物质的含量与碱胁迫过程中碱浓度之间的关系。用不同浓度的Na2CO3溶液进行花生幼苗的处理，共设计了五个不同浓度的 Na2CO3溶液，分别为 0mmol·L-1、12.5mmol·L-1、25mmol·L-1、37.5mmol·L-1、50mmol·L-1[1]。花生幼苗在碱胁迫条件下处理的时间分别为 0 天、1 天、8天、9 天、14 天，胁迫后测量叶片中各个渗透调节物质，如可溶性糖(SS)、可溶性蛋白质、游离脯氨酸(Pro)和膜过氧化产物丙二醛(MDA)的含量。由实验结果可以知道，随着碱胁迫浓度的升高，花生叶片渗透调节物质,如可溶性糖、可溶性蛋白质、游离脯氨酸和丙二醛的含量都有显著增加；而且随着碱胁迫时间的延伸，花生幼苗渗透调节物质含量也有显著增长。该论文有图7 幅，有表3 幅，参考文献15 篇。51006 毕业论文关键词：碱胁迫 花生叶片 渗透调节物质 Effects of alkali stress on Osmotic AdjustmentSubstances of peanut leavesAbstractThe experimental materials of this experiment are the peanut seed.Theexperiment was used to explore the relationship between the content of osmoticadjustment substances in the leaves of peanut and the alkali concentration under thealkali stress.In this experiment,the peanut seedlings was treated with differentconcentrations of Na2CO3 solution, and a total of five levels of Na2CO3 solution weredesigned.The concentrations were 0mmol·L-1,12.5mmol·L-1, 25mmol·L-1, 37.5mmol·L-1, 50mmol·L-1 respectively.Peanut seedling alkali stress treatment time was 0days,1 days,8 days,11 days,14 days, various osmotic adjustment substances, such assoluble sugar (SS), soluble protein, free proline (Pro) and membrane lipid peroxidation product malondialdehyde (MDA) content was determined.Theexperimental results showed that with the increase of the concentration of alkali,soluble sugar, soluble protein, free Pro and MDA content in peanut leaves weresignificantly increased.And with the extension of stress time, the content of osmoticadjustment substances of peanut seedlings also increased significantly. Key words:Alkali stress Peanut leaves Osmotic adjustment substance 目录 摘要.......1 Abstract...... 1 目录.......3 图清单.. 4 表清单.. 4 0 绪论.. 5 1 材料与方法......6 1.1 供试材料6 1.2 材料处理6 1.3 分析测定方法....... 6 1.4 数据处理...... 9 2 结果与分析......9 2.1 碱胁迫对花生叶片可溶性糖(SS)含量的影响....... 9 2.2 碱胁迫对花生叶片可溶性蛋白质含量的影响..... 10 2.3 碱胁迫对花生叶片游离脯氨酸(Pro)含量的影响.......10 2.4 碱胁迫对花生叶片丙二醛(MDA)含量的影响....... 11 3 讨论 12 参考文献....13
致谢.....14 |