碱胁迫对大豆叶片渗透调节物质的影响研究
时间:2020-06-17 20:50 来源:毕业论文 作者:毕业论文 点击:次
摘要 研究不同浓度Na2CO3 胁迫对大豆幼苗叶片中渗透调节物质(可溶性糖、可溶性蛋白质、游离脯氨酸含量)和丙二醛含量指标的影响。用 0.15%、0.30%、0.45% 的Na2CO3 溶液对大豆苗进行胁迫处理, 同时以不含Na2CO3 的完全培养液作为对照,测定相关生理指标的变化。结果表明:大豆幼苗叶片生理特性随着Na2CO3 胁迫浓度增大和胁迫时间延长而发生了明显的变化:渗透调节物质(可溶性糖、可溶性蛋白质、游离脯氨酸)和丙二醛含量在一定范围内显著提高。综合比较可得大豆可以通过增加渗透调节物质来适应适度的碱胁迫, 但高浓度加上长时间的碱胁迫会对大豆造成严重损伤。 51087 毕业论文关键词:大豆 Na2CO3 碱性盐胁迫 渗透调节物质 丙二醛 Alkali stress on the influence of soybean leaf osmotic regulation substances research Abstract The effect of different concentrations (0.15%, 0.30%, 0.45% of Na2CO3) of Na2CO3 stress on osmotic regulation substances in the leaves of soybean seedlings (Soluble sugar, soluble protein, free proline content) and malondialdehyde content in this paper was studied, with no Na2CO3 culture as a reference. The related physiological indexes were determined. The results showed that with the increase of stress level and the extension of time, significant changes have taken place in physiological characteristics of soybean seedling leaf: osmoregulation substance (soluble sugar, soluble protein and free proline) and malondialdehyde content within a certain range increased significantly. It is available for soybean by increasing osmotic regulation substances to adapt to a moderate amount of alkali stress, but high concentration and long time of alkali stress can cause serious damage to soybean. Key Words: soybean Na2CO3 alkaline salt stress Osmotic regulation substances malondialdehyde 目录 摘要.4 关键词:..4 目录6 引言..7 1材料与方法..8 1.1试验材料8 1.2测定方法8 1.3数据处理8 2结果与分析..8 2.1大豆叶片渗透调节物质和丙二醛在Na2CO3碱性盐胁迫下的含量变化分析.8 3讨论与结论.12 3.1讨论...12 3.2结论...14 参考文献.15
致谢.17 |