拟南芥AtRDR1过量表达转基因的鉴定与分析
时间:2020-06-17 21:00 来源:毕业论文 作者:毕业论文 点击:次
| 摘要雌雄异位，同一朵花的雌雄生殖器在空间上的分离， 是一种可以增加异交率的变异表型。有研究表明，rdr6（RNA-dependent RNA polymerase6）突变体能增强拟南芥花的自交不亲和，促进柱头伸长。目前在绝大多数的植物中，雌雄异位的分子调控机制还未得到详细阐述。本研究把拟南芥这种植株花朵较小且高度自交的模式生物作为研究对象。阐述了利用侵染花序的方法将拟南芥At1g14790 和At1g14840基因转染到拟南芥 Col-0中， 待转基因拟南芥种子长成，利用 PCR 扩增法检验是否存在所导入的基因，并观察花朵是否存在雌雄异位表型。但是由于环境以及种植过程中出现了问题，导致实验结果不充分，不能很好地体现 AtRDR1基因的过量表达对拟南芥雌雄异位表型的影响。从现有的数据来看，At1g14840 的过量表达与雌雄异位表型的存在有一定关系，大部分成功导入At1g14840的植株存在雌雄异位表型。该论文有图5 幅，表5 个，参考文献12 篇。51090 毕业论文关键词：雌雄异位 依赖于RNA 的 RNA 聚合酶 转基因 PCR 扩增 Identification and Analysis of Arabidopsis thalianaRDR1 gene overexpressing Abstract Herkogamy, the spatial separation of male and female reproductiveorgans within flowers, it is a way to increase outcrossing rate as a variablephenotypic. Studies have shown that, RDR6 (RNA-dependent RNApolymerase6) mutant of Arabidopsis flower can enhance self-incompatibilityand promote stigma elongation. We make Arabidopsis thaliana as conesearchsubjects, a model plant with small flower and highly selfing. The articledescribes that infecting inflorescence method is utilized to infect AtRDR1 geneAt1g14790 and At1g14849 into the Arabidopsis thaliana Col-0. Whentransgenic Arabidopsis thaliana seeds has grown,using PCR amplificationmethod to verify the presence of the transfered gene,and observe weather thereare the herkogamy phenotype in the flower.However, due to environment andthe problems in the process of planting, the results are not sufficient, can notproperly reflect the impact of overexpression of AtRDR1 gene on appearanceof herkogamy phenotype.From the existing data, the overexpressing ofAt1g14840 and the presence of herkogamy variant have a certainrelationship,most Arabidopsis thaliana successfully transfering At1g14840exist herkogamy phenotype. Key words:herkogamy RNA-dependent RNA polymerases transgenosisPCR amplification 目录 摘要.Ⅰ Abstract.Ⅱ 目录.Ⅲ 图清单Ⅳ 表清单Ⅳ 1绪论1 1.1 交配模式对进化的意义.. 1 1.2 雌雄异位性状及其生态学意义..1 1.3 拟南芥作为模式植物的优点..2 1.4 拟南芥RDR（RNA-dependent RNA polymerases）基因相关研究进展.2 2 实验材料与方法. 3 2.1本实验所用材料：3 2.2 实验方法5 3 结果与分析.. 12 3.1 雌雄异位表型及其分析 12 3.2 拟南芥基因组DNA 提取与浓度测定 14 3.3 PCR 鉴定转基因插入子.15 3.4 统计结果. 17 4 讨论 19 4.1对于实验结果的讨论. 19 4.2对拟南芥的温室种植和培育的讨论 19 参考文献20
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