转OsSTSL2基因在逆境胁迫下的生理分析

摘要

水稻（Oryza sativa L。）是世界上主要粮食作物之一，然而随着近年来的人口增长、环境污染和全球变暖等因素的影响，水稻越发受到盐碱胁迫和干旱胁迫，对水稻的生长发育和产量造成了严重影响。因此，研究和开发相关的水稻抗盐、抗旱等抗逆基因并利用分子生物学技术对水稻进行遗传改良，提高水稻抗逆性有着重要意义。利用本实验室前期获得的转OsSTSL2基因水稻材料进行盐胁迫和干旱胁迫的基因功能研究。在150 mM NaCl和15% PEG 6000模拟干旱处理下，观察表型发现过表达株系叶片和根系的生长状况在逆境胁迫下要好于日本晴，说明OsSTSL2基因的过表达株系能够较好的适应胁迫环境。分析逆境下转OsSTSL2基因水稻的抗氧化酶含量变化，发现过表达株系叶片的CAT酶、SOD酶和POD酶活力较明显的高于同时期的日本晴和RNAi植株，这些结果都表明转OsSTSL2基因水稻有一定的抗氧化防御系统能力。85150

Abstract

Rice (Oryza sativa L。) is one of the world's major food crops。 However, in recent years, with some negative factors such as population growth, environmental pollution and global warming, the growth of rice is damaged seriously by salt and drought stress。 Therefore, it is of great significance to study and develop the related genes such as salt tolerance and drought resistance of rice, and to improve the resistance of rice by genetic modification using molecular biology technology。In our lab, the transgenic rice plants were screened at early stages and used to be materials for study of gene function under salt stress and drought stress。 With the treatment of 150 mM NaCl and 15% PEG 6000, it was found that the leaf and root growth of overexpression lines was better than that of Japonica and RNAi plants。 The results indicated that the overexpression of OsSTSL2 gene in rice could be adapted to the stress environment。 Based on the changes of antioxidant enzyme activities of OsSTSL2 transgenic rice plants under stress, it was found the activities of CAT enzyme, SOD enzyme,and POD enzyme of overexpression plants were significantly higher than that of japonica and RNAi plants in the same period, These results suggested that OsSTSL2 transgenic rice had the capability of antioxidant defense。源Q于W优H尔J论K文M网WwW.youeRw.com 原文+QQ75201.,8766

毕业论文关键词：水稻；盐胁迫；干旱胁迫；OsSTSL2基因；抗氧化

Key words: rice; salt stress; drought stress; OsSTSL2 gene; antioxidant

1。材料和方法 4

1。1。供试材料 4

1。1。1。水稻材料 4

1。1。2 4

1。2。试验处理 5

1。3。水稻生理数据的测定 5

1。3。1。叶绿素含量 5

1。3。2。酶活力 5

1。4。数据处理 6

2。结果和分析 6

2。1。叶片叶绿素含量变化 6

2。2。盐胁迫下转OsSTSL2基因水稻酶含量变化 7

2。3。干旱胁迫下转OsSTSL2基因水稻酶含量变化 9

3。讨论 11

人类社会经济的快速发展、人口的急速膨胀和生态环境的不断恶化，造成了干旱、盐碱、极端温度、重金属污染等灾害的日趋加重，尤其在我国特别严重，这些逆境因子极大地影响了作物的生长发育，进而影响了作物的产量和品质。粮食问题逐渐成为全球性问题之一，水稻又是最重要的粮食作物之一，全世界超过一半的人口以其作为主食。我国超过65%的人口以稻米为食[1]。外界因素极大地影响水稻的产量，如逆境胁迫--盐胁迫、干旱胁迫等。来自优W尔Y论W文C网WWw.YoueRw.com 加QQ7520,18766 转OsSTSL2基因在逆境胁迫下的生理分析:http://www.youerw.com/shengwu/lunwen_196553.html