摘要：拟南芥(Arabidopsis thaliana)是十字花科拟南芥属，由于它具有植株小，结构简单，基因组小，生长周期短，繁殖系数高，容易进行遗传转化等特点，因此成为遗传、细胞、发育、分子生物学研究的模式生物。在遗传学研究方面有着重要的作用。拟南芥的全基因组序列测定工作已于2000年12月完成。为此以植物拟南芥为材料来研究GPDH基因的功能，GPDH是油脂合成过程中的重要酶，具有提高油脂含量的功能，在脂肪酸合成途径，TAG合成途径以及抵抗渗透胁迫能力提高植物的免疫力等方面有重要的作用，为进一步研究GPDH的生物学方面的功能，本文利用生物信息学的方法对其蛋白质的理化性质、不同物种的蛋白质的同源性及进化树分析、亚细胞定位、保守结构域、信号肽、蛋白质的高级结构、基因的表达情况等进行预测和分析，结果表明各种AtGPDH的相对分子质量和氨基酸的含量差异有一定的差异，同源性及进化树分析表明拟南芥与甘蓝型油菜的同源性最高且亲缘关系最近。GPDH蛋白没有跨膜结构域和信号肽，拟南芥GPDH在不同的组织和器官有不同的表达，其中在种子的表达最强。本研究的结果将为进一步的研究拟南芥的GPDH的生物学功能提供参考依据。27864
毕业论文关键词：拟南芥； GPDH ； 油脂合成 ； 表达分析 ； 生物信息学
Bioinformatics Analysis of the Gene of 3- Glycerol Dehydrogenase（AtGPDH）from Arabidopsis thaliana
Abstract：Crucifer Arabidopsis Arabidopsis (Arabidopsis thaliana), because of its small plants, has the advantages of simple structure, small genome, short growth period, high propagation coefficient, easy genetic transformation characteristics. Therefore, it has become genetic, cellular, developmental, molecular biology studies of model organisms. It has important function in genetics research.The whole genome sequence of Arabidopsis was completed in December, 2000. This plant Arabidopsis was used to study the function of GPDH gene, GPDH is an important enzyme in the synthesis process of oil, improve oil content has the function, in the glycerol synthesis pathway, the fatty acid synthesis pathway, TAG pathway and resistance to osmotic stress and improving the immunity of plants has an important role, for further biological study on the function of GPDH, using bioinformatics methods on protein physicochemical properties of different species, protein homology and phylogenetic tree analysis, subcellular localization, conserved domain, signal peptide, protein structure, gene expression level were predicted and analyzed, results show that there are certain differences the difference of content of relative molecular mass and various amino acids AtGPDH, homology and phylogenetic analysis showed that recently, Arabidopsis thaliana and Brassica napus highest homology and phylogenetic relationship. The GPDH protein had no transmembrane domain and signal peptide, and the expression of GPDH in different tissues and organs was different, and the expression of the seed was the strongest in the seed.. The results of this study will provide a reference for further studies on the biological function of GPDH in Arabidopsis.
Keywords: Arabidopsis ；GPDH ; Oil synthesis ; expression anaiysis ; bioinformatics
目 录
摘要 4
引言 6
1材料 6
1.1生物信息学数据库与软件 6
2分析方法 7
2.1蛋白质序列的获取 7
2.1.1拟南芥G3PDH基因的蛋白质序列的获得 7
2.1.2拟南芥GPDH蛋白的进化分析 7
2.1.3同源序列的获得 7
2.1.4同源序列的对比及系统发育树分析 7 拟南芥3-磷酸甘油脱氢酶（AtGPDH）基因的生物信息学分析:http://www.youerw.com/shengwu/lunwen_22511.html