摘要：本实验基“黄金芽”转录组数据，通过PCR方法从“黄金芽”中得到GGT1和GGT3基因，其中CsGGT1和CsGGT3基因分别含有1801和1861 bp的开放阅读框，编码599和619个氨基酸。从进化树、亲/疏水性、无序化特性、二级结构等方面进行预测与分析，结果表明大多数氨基酸属亲水性氨基酸，无序化特征比较明显，利用实时荧光定量 PCR 分析表明，在迎霜、安吉白茶、黄金芽 3 个茶树品种中 GGT1、GGT3 基因在茶树的不同组织表达存在明显差异，其中在3rd叶片中表达水平最高，而黄金芽在这三个品种中表达水平最高。GGT1和GGT3均受高温、低温诱导表达。其中GGT1在低温、高温均受抑制。GGT3在低温表达水平提高，在高温时受抑制。说明GGT基因在茶树温度胁迫中调控中存在作用。28059
毕业论文关键词：茶树；GGT；基因克隆；温度胁迫
Cloning and tissue stress of γ - glutamyl transpeptidase gene in A study of differences in expression
Abstract：The GGT1 and GGT3 genes were obtained from the "Huangjinya" by PCR, and the CsGGT1 and CsGGT3 genes contained 1801 and 1861 bp open reading frames, encoding 599 and 619 amino acids, respectively. The results showed that most of the amino acids were hydrophilic amino acids and the disorder characteristics were obvious. The results of real-time fluorescence quantitative PCR showed that the amino acids were not sensitive to the amino acids, There were significant differences in the expression of GGT1 and GGT3 genes in tea leaves, and the expression level of GGT1 and GGT3 in tea leaves was the highest in the 3rd leaf, and the expression level of GGT1 and GGT3 was the highest among the three cultivars. GGT1 and GGT3 were induced by high temperature and low temperature. Which GGT1 in low temperature, high temperature are inhibited. GGT3 increased at low temperature and inhibited at high temperature. Indicating that GGT gene plays an important role in the regulation of tea plant temperature stress.
Key words: Tea tree; GGT; gene cloning; temperature stress
目 录
摘要 1
关键词 1
Abstract 1
Key words 2
引言（或绪论） 2
1 材料与方法 2
1.1 植物实验材料 2
1.2总RNA提取 2
1.3总RNA的纯化及检测 3
1.4cDNA合成 3
1.5茶树CsGGT1和CsGGT3基因的克隆 4
1.6pMD18-T Vector连接 4
1.7生物信息学分析 6
2.实验结果 6
2.1茶树CsGGT1与CsGGT3基因 6 2.2 茶树CsGGT1和CsGGT3的进化分析 9
2.3 茶树 CsGGT1和CsGGT3 转录因子推导氨基酸疏/亲水性和无序化分析 9
2.4 茶树 CsGGT1和Cs转录因子的二级结构预测与分析 9
2.5茶树 CsGGT1和CsGGT3基因的表达 10
3.讨论 15
致谢 16
参考文献 16
图1 不同物种GGT1和GGT3转录因子的系统进化树 10
图2 茶树 CsGGT1和CsGGT3 氨基酸序列疏水、亲水性质分析 12
图3 茶树 CsGGT1折叠状态的分析 12
图 4 茶树CsGGT3折叠状态的分析 13 茶树γ-谷氨酰转肽酶基因的克隆及其组织逆境表达差异的研究:http://www.youerw.com/shengwu/lunwen_22769.html