摘要：以天然生物防腐剂作为化妆品防腐剂的研发应用已成为当前的行业发展趋势。聚赖氨酸(ε-poly-L-lysine,ε-PL)作为生物防腐剂，具有抑菌谱广、水溶性好、无毒副作用 、安全性高等特点。本课题考察了聚赖氨酸对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌及黑曲霉的抑菌性能，确定了相应的MIC值，并进行了化妆品防腐效力测试。实验结果表明：聚赖氨酸对细菌的抑菌效果优于化妆品常用防腐剂尼泊金甲酯，聚赖氨酸对真菌的抑菌效果略差于尼泊金甲酯，课题优化了防腐剂体系，提出采用聚赖氨酸(ε-PL)与尼泊金甲酯按3:1配比，按0.05%添加量使用，在保证化妆品防腐体系安全性的前提下，为聚赖氨酸(ε-PL)作为天然防腐剂部分替代化学防腐剂尼泊金甲酯的应用，提供理论与实践依据。5567
关键字： 聚赖氨酸；尼泊金甲酯；化妆品；抑菌效果;毕业论文
Analysis of ε-Polylysine as a cosmetic preservative Feasibility
Abstract：The use of natural biological preservative is becoming the current research and development trend for cosmetic preservative. The advantages of polylysine (ε-poly-L-lysine, EPL) as a biological preservative include broad inhibition spectrum, high water-solubility, no toxic side effects and high safety. This study examines the antibacterial properties of polylysine against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Aspergillus niger; determines the corresponding MIC values; and tests cosmetic preservative efficacy. The experimental results show that: while polylysine has superior antibacterial compares to commonly used cosmetic preservative agent methylparaben, its inhibitory effect on fungi is slightly worse. To optimize preservative effect, the study proposes the use a mixture of polylysine and methylparaben in 3:1 ratio by weight at 0.05%. The study provides theoretical and practical basis for polylysine to be used as a natural alternative to chemical preservatives such as methylparaben while maintaining cosmetic preservative safety.
Keywords: Polylysine; Methylparaben; Cosmetics; Bacteriostatic effect
目 录
1 绪论 1
1.1 化妆品防腐剂简介 1
1.1.1化妆品用防腐剂的要求 1
1.1.2化妆品防腐剂使用现状 1
1.2 聚赖氨酸简介 2
1.2.1 聚赖氨酸的结构 2
1.2.2 聚赖氨酸的理化性质 3
1.2.3 聚赖氨酸的抑菌机理 3
1.2.4 聚赖氨酸的生物学性质 3
1.2.5 聚赖氨酸的发酵制备 4
1.2.6聚赖氨酸作为防腐剂国内外研究现状 4
1.3本课题研究的主要内容 4
2.1 材料 5
2.1.1 供试菌种 5
2.1.2 培养基和主要试剂 5
2.1.3 主要器材设备 6
2.2 方法 6
2.2.1供试菌样制备： 6
2.2.2试剂制备 6
2.2.3 测试平皿制作 6
2.2.4 滤纸片法测定防腐剂抑菌效果 6
2.2.5 MIC的确定 7
2.2.6供试化妆品的制备 7
2.2.7防腐剂效力测试 8
3 实验结果与分析 9
3.1防腐剂抑菌效果实验观察结果 9
3.1.1 防腐剂对大肠杆菌的抑菌效果 9
3.1.2 防腐剂对金黄色葡萄球菌的抑菌效果 10
3.1.3 防腐剂对铜绿假单胞菌的抑菌效果 10
3.1.4 防腐剂对黑曲霉的抑菌效果 11 聚赖氨酸作为化妆品防腐剂可行性分析:http://www.youerw.com/shengwu/lunwen_2640.html