百萨偃麦草6J染色体特异标记开发及在黑麦6R上可转移性分析

摘要：百萨偃麦草（Thinopyrum bessarabicum Love,2n=2x=14, JJ）具有较高的耐盐性和兼抗多种麦类病害的特点，可以作为改良小麦产量和品质的重要基因资源。百萨偃麦草6J染色体上可能含有耐盐相关基因，且6J染色体短臂易位系具有较高的锌铁含量，因此推测6J染色体短臂上具有控制锌铁含量的相关基因。本试验根据百萨偃麦草转录组序列开发了132对6J专化引物并在中国春、中国春-百萨偃麦草双二倍体、中国春-百萨偃麦草6J染色体二体添加系DA6J和6J染色体二体代换系DS6J(6A)上进行扩增，发现能扩增出特异条带的有46对，其中10对在普通小麦中国春6A染色体也具有特异扩增位点，因此这些标记可以共显性标记同时检测染色体6J和6A；将筛选出的46对6J特异标记引物在普通小麦地方品种辉县红、荆州黑麦、辉县红-荆州黑麦双二倍体荆辉1号和DA6R中进行扩增，结果表明，46对6J特异标记引物中有8对在黑麦6R上可以扩增出多态性位点，可以作为6R的特异标记，并说明百萨偃麦草6J与黑麦6R间存在部分同源性和基因保守性。36606
毕业论文关键词：百萨偃麦草；6J染色体；可转移性；特异标记
Development and Identification of Specific Markers for Chromosome 6J of Thinopyrum bessarabicum and Analyzing the Transferability in Chromosome 6R of Secale cereal
Abstract：Thinopyrum bessarabicum (2n = 2x = 14, JJ) possesses salinity tolerance and resistance to several diseases, and can be an important gene source for wheat improvement. There are many favorable genes on chromosome 6J such as salt tolerance, high content of zinc, iron. In this paper, 132 pairs of primers designed according to the transcriptome sequences mapped on group 6 chromosomes of wheat were used to amplify in wheat Chinese Spring, Chinese Spring - Thinopyrum bessarabicum amphiploid. Chinese Spring- Th. bessarabicum addition line DA6J, Chinese Spring- Th. substitution line DS6J(6A)，46 chromosome 6J specific makers were selected including 11 are co-dominance which could detect both chromosome 6A and 6J. 46 pairs of primers specific amplified in 6J were used to amplify in wheat landrace Huixianhong, rye, Huixianhong-Jingzhouheimai amphiploid JH1, Huixianhong-Jingzhouheimai addition line DA6R, the results showed that 8 pairs also produced specific loci of rye indicating the high transferability of Th. bessarabicum markers in rye, this results meant that chromosomes 6J and 6R shared high collinearity.
Key words：Thinopyrum bessarabicum; Chromosome 6J; Transferability; Specific markers
目录
摘要3
关键词3
Abstract3
Key words3
引言3
1材料与方法4
1.1材料 4
1.2方法 5
1.2.1 DNA的提取5
1.2.2 PCR的扩增5
1.2.3扩增产物的检测5
2结果与分析5
2.1 百萨偃麦草6J染色体特异标记的开发与鉴定5
2.2百萨偃麦草6J染色体特异标记在黑麦6R上可转移性分析 7
3讨论9
致谢10
参考文献11
百萨偃麦草6J染色体特异标记开发及在黑麦6R上可转移性分析
引言：百萨偃麦草（Thinopyrum bessarabicum Love, 2n=2x=14, JJ）是多年海生小麦属植物，具有较高的耐盐性和兼抗多种麦类病害的特点，可以作为改良小麦产量和品质的重要基因资源[1~3]。前人研究发现，百萨偃麦草4J染色体上含有控制籽粒呈蓝色的基因，并通过分子标记技术将蓝粒基因定位在4JL-11区域[4]，该基因可以作为表型选择的工具，并对小麦营养品质的改良具有重要作用。百萨偃麦草染色体1J含有HMW-GS基因，可有效改良小麦的加工品质[5]。小麦-百萨偃麦草补偿易位系T2JS-2BS.2BL对增加小麦单株产量、株高、分蘖、穗长和穗粒数具有重要作用，但同时会使小麦的抽穗期延迟[6]。百萨偃麦草6J染色体上可能含有耐盐相关基因[7]，且6J染色体短臂易位系具有较高的锌铁含量，因此推测6J染色体短臂上具有控制锌铁含量的相关基因[8]。百萨偃麦草6J染色体特异标记开发及在黑麦6R上可转移性分析:http://www.youerw.com/shengwu/lunwen_35125.html