根瘤菌肥对大豆盐胁迫响应基因mRNA表达水平的影响研究

摘要大豆是重要的经济作物，盐害却严重影响了其产量。前期研究表明，类黄酮代谢途径在大豆耐盐胁迫机制中扮演重要角色，而根瘤菌与豆科植物共生过程中此类代谢物质亦是极为关键。为了探索提高大豆耐盐胁迫能力的新方法，本研究以苜蓿中华根瘤菌Rm1021为菌肥，应用于大豆Union85140幼苗期。然后通过荧光定量PCR检测方法，从大豆耐盐响应的Ca2+信号转导途径基因(GmCAx)、查尔酮代谢途径关键酶基因(GmCCx)、转录因子MYB家族基因(GmMYBx)、过氧化物清除途径关键酶基因(GmROSx)等几个方面定量分析大豆根的mRNA表达量。结果显示，Glyma04g39930.1、 Glyma12g03610.1、Glyma07g16910.1、Glyma14g03470.1、Glyma10g33650.1、Glyma11g15680.5、 Glyma14g39810.1、Glyma17g38140.1、Glyma12g07780.3、Glyma15g02450.1、Glyma07g37240.2、Glyma06g02330.1、Glyma04g0227.1、Glyma19g40820.1、Glyma09g05440.1、Glyma07g14460.1 、Glyma08g11520.1、Glyma08g11610.1、Glyma08g11530.1、Glyma01g43880.1、Glyma08g11635.1、 Glyma08g11630.2、Glyma08g11620.1、Glyma05g28610.2、Glyma04g40030.1、Glyma06g14820.1、 Glyma01g40220.1及Glyma08g20270.1等基因呈上调趋势，Glyma20g33880.2、Glyma11g19840.2、Glyma12g08650.1、Glyma12g30260.1、Glyma10g33710.1、Glyma20g38980.2、Glyma10g01200.1、Glyma13g34100.1及Glyma08g19270.1等基因呈下调趋势。这些结果表明，根瘤菌能通过过氧化物清除途径、查尔酮/类黄酮代谢等途径调节大豆耐盐能力。47801

毕业论文关键词：大豆；耐盐；分子标记；mRNA

Abstract Soybean is an important economic crop, but its yield is seriously affected by salt stress. Previous studies showed that flavonoid metabolism pathway played an important role in the mechanism of salt tolerance in soybean. The flavonoids are well known as key factors in symbiosis process between rhizobium and legumes. In order to explore a sustainable way for improving the soybean tolerance to salt stress, the medicago Sinorhizobium meliloti was used to infect the soybean (Cultivar Union85140) seedlings. After the S. meliloti infection, the Union85140 seedlings were then transferred in sphagnum peat and pearlite soil with 0, 100, 200 and 300 mM NaCl. The roots were harvest at three-trifoliate stage for quantitative real-time PCR analysis. The tested salt responsive genes were involved in Ca2+ signal transduction pathway, chalcone metabolic pathway, MYB transcription factors and the ROS scavenging pathway. Results showed that, the genes of Glyma04g39930.1, Glyma12g03610.1, Glyma07g16910.1, Glyma14g03470.1, Glyma10g33650.1, Glyma11g15680.5, Glyma14g39810.1, Glyma17g38140.1, Glyma12g07780.3, Glyma15g02450.1, Glyma07g37240.2, Glyma06g02330.1, Glyma04g0227.1, Glyma19g40820.1, Glyma09g05440.1, Glyma07g14460.1, Glyma08g11520.1, Glyma08g11610.1, Glyma08g11530.1, Glyma01g43880.1, Glyma08g11635.1, Glyma08g11630.2, Glyma08g11620.1, Glyma05g28610.2, Glyma04g40030.1, Glyma04g40030.1, Glyma06g14820.1, Glyma01g40220.1 and Glyma08g20270.1 were up-regulated. Glyma20g33880.2, Glyma11g19840.2, Glyma12g0865

0.1, Glyma12g30260.1, Glyma10g33710.1, Glyma20g38980.2, Glyma10g01200.1, Glyma13g341

0.1, and Glyma08g19270.1 were down-regulated. These results indicate that rhibium infection could improve the salt tolerance in soybean and this function might regulated by chalcone/flavonid meteabolism pathway.

Keyword: Soybean; salt tolerance; molecular marker; mRNA

1大豆春化、灭菌、萌发 7

1.1材料和方法 7

1.1.1试验材料 7

1.1.2实验方法 7

2大豆根总RNA提取 7

2.1材料和方法 7

2.1.1实验材料根瘤菌肥对大豆盐胁迫响应基因mRNA表达水平的影响研究:http://www.youerw.com/shengwu/lunwen_50023.html