反硝化细菌培养条件研究

摘要好氧反硝化细菌可以克服传统的缺氧反硝化工艺的缺陷，能够在好氧的条件下进行反硝化作用，在污水脱氮处理中有着很强的应用潜力。本文采用酸碱指示剂法和滴加试剂法，从自然环境中筛选得到1株好氧反硝化细菌（ADB5），显微镜下形态为杆状。该株细菌在筛选实验中，杜氏小管中产气量为1.8ml，硝态氮的去除率达82%。为了更好地了解并利用该细菌，从碳源、氮源、碳氮比、培养温度、初始pH等因素着手，用单因素法探讨了好氧反硝化细菌生长的最佳环境条件。结果显示，培养ADB5最佳氮源为蛋白胨，碳源为柠檬酸钠，C/N质量比为6g/4g，初始培养基pH为7，培养温度为30℃。63954

毕业论文关键词好氧反硝化筛选硝酸盐亚硝酸盐降解

毕业设计论文外文摘要

Title Study on Optimum Culture Conditions of Denitrifying Bacteria

Abstract Aerobic denitrification bacteria can overcome the defects of traditional anoxic denitrification process and can denitrify in aerobic conditions. It has a great potential for application in wastewater denitrification. A aerobic denitrification bacteria(named ADB5) was screened from the natural environment by acid-base indicator method and dropping reagent method in the paper. Its basic shape was bacilli under microscope. During the process of screening strains, gas production of ADB5 was 1.8ml in Duchenne tubules and its nitrate removal rate reached 82%. In order to understand and use the bacteria better, we optimized culture conditions of the aerobic denitrifying bacteria by a single factor method. Carbon, nitrogen, C/N ratio, cultivation temperature and initial pH of the media were studied here. Results showed the best nitrogen source was peptone and carbon source is sodium citrate for ADB5. The C/N ratio of the mass is 6g/4g, the initial pH of the media is 7, cultivation temperature is 30℃.

Keywords Aerobic denitrifier Screening Nitrate Nitrite Degradation

1 引言 1

1.1 氮元素的来源及危害 1

1.2 氮素去除方法 1

1.3 生物脱氮技术 2

1.3.1传统理论上的生物脱氮工艺 2

1.3.2 同步硝化反硝化(SNdN)过程 2

1.3.3 短程硝化-反硝化脱氮技术 2

1.3.4好氧反硝化脱氮技术 3

1.4 好氧反硝化细菌的筛选方法 4

1.5 好氧反硝化细菌生长的影响因素 5

1.5.1 碳源、氮源 6

1.5.2 碳氮比 6

1.5.3 pH值 6

1.5.4 温度 7

1.6 好氧反硝化作用机理 7

1.7 本课题研究目的及内容 7

2 好氧反硝化细菌的筛选及鉴定 9

2.1 材料与方法 9

2.1.1 菌种来源与培养基 9

2.1.2试剂、仪器设备 10

2.1.3 细菌的筛选 11

2.1.4水体中不同形态氮含量的测定 12

2.1.5降解率计算方法反硝化细菌培养条件研究:http://www.youerw.com/shengwu/lunwen_70827.html