摘要【目的】针对细菌感染与细菌生物被膜有关，在体外建立细菌生物被膜形成模型，并对形成的细菌生物被膜检测选择比较稳定的做破坏试验。【方法】在96-孔微量培养板中制备细菌生物被膜，结晶紫染色来量化细菌生物被膜在不同条件下的形成情况，扫描电镜对形成的细菌生物被膜形态进行分析。【结果】铜绿假单胞菌在48小时才能形成生物被膜在静态条件下形成的生物被膜较稳定，抗生素对它有破坏作用【结论】结晶紫染色能半定量细菌生物被膜的形成，扫描电镜能很好观察细菌生物被膜形成与破坏。8789
由于抗生素的滥用，新临床发现的多重耐药菌株越来越多，细菌生物被膜的形成是导致多重耐药的第一道屏障和主要原因之一。在细菌生物被膜的保护下，细菌可以增强自身对抗生素、环境压力及宿主免疫系统攻击的耐受能力。本课题立足于铜绿假单胞菌生物被膜形成的基础上利用本课题组分离出的天然药物单体对生物被膜的抑制和破坏，进而达到抑菌的目的。
关键词 铜绿假单胞菌 生物被膜 抗药性 扫描电镜 形成 抑制
Abstract: [Objective] Bacterial infection is relative to bacterial biofilm formation, thus we established bacterial biofilm formation model in vitro, and detected the formed bacterial biofilm. [Methods] We prepared bacterial biofilm in the 96-well polystyrene microtitre plates, crystal violet staining was quantified bacterial biofilm in various conditions, and Scanning Electron Microscopy (SEM) was analysed the morphology of bacterial biofilms. [Results] the remaining four kinds of bacteria can form biofilm for 48h. Pseudomonas aeruginosa biofilms were more stable in static condition, [Conclusion] Crystal violet staining was used for semiquantitation bacterial biofilms formation, and SEM can be devoted to observing the morphology of bacterial biofilms.
Due to abuse of antibiotics, more and more new multi-resistant clinical bacterial strains have been found in the recent decades. Bacterial biofilm is the first barrier of multiple-drug resistant,of which formation is one of the main reasons that lead to resistance.Under the protection of the biofilm,the bacteria can enhance their own antibiotic, pressure on the environment and attack tolerance of the host immune system . Based on the P. aeruginosa biofilm formation, suppression and disruption of the biofilm by some natural product are to inhibitate the corresponding bacteria in this topic.
Keyword Pseudomonas aeruginosa Biofilm Resistance Scanning electron microscopy
Formation Suppression
目录
1.前言 2
1.1铜绿假单胞菌简介 3
1．2 铜绿假单胞菌研究的临床意义 4
1．3 铜绿假单胞菌的耐药性 5
1.4铜绿假单胞菌耐药机制 6
1.5细菌生物膜感染对患者的免疫损害 7
2.生物被膜的形成 8
2.1铜绿假单胞菌被膜的形成 9
2.2铜绿假单胞菌被膜的形成实验 10
2.2.1实验材料 10
2.2.2试验方法： 10
2.3实验结果 11
3.生物被膜的破坏 12
3.1铜绿假单胞菌生物被膜形成的影响因素 13
3.2抗生素对铜绿假单胞菌生物被膜形成的影响 13
3.3铜绿假单胞菌生物被膜破坏试验 14
3.3.1实验材料 14
3.3.2试验方法： 15
3.4实验结果 15
参考文献 16
1.前言
铜绿假单胞菌为条件致病菌，当机体免疫力低下时可引起多种感染。近年来，对人的致病作用明显增加，成为一系列严重的化脓性感染，尤其是支气管扩张、慢性支气管炎、囊性肺纤文化等基础疾病继发感染的重要致病菌。随着抗菌药物的广泛应用，细菌耐药性越来越严重，而且有明显上升趋势，给临床治疗造成极大困难。 铜绿假单胞菌被膜的形成与破坏:http://www.youerw.com/shengwu/lunwen_7248.html