亚硝酸盐污染生物处理工艺设计

摘要：亚硝酸盐摄入过量就会对人体产生很大的伤害。本课题主要通过研究几种乳酸菌对亚硝酸盐的反应，探讨时间对乳酸菌降解亚硝酸盐的影响。分别将分离纯化号的乳酸菌H和乳酸菌F接种到MRS培养基中。在反应12小时后，用分光光度计测其在波长600nm下的OD值.在OD值在0.8左右时，加入1ml 0.2g/ml的亚硝酸盐.运用盐酸萘乙二胺法，用紫外分光光度计测定其在波长538nm下，不同反应时间时的亚硝酸盐的OD值,并用实验室PH计测不同反应时间段后的PH值。71220

毕业论文关键字：乳酸菌; 亚硝酸盐; 降解; 盐酸萘乙二胺法

Biological treatment of nitrite pollution

Abstract: Excessive intake of nitrates can cause great harm to humans. In this study, we studied the reaction of several lactic acid bacteria to nitrite, and investigated the effect of time on the degradation of nitrite by lactic acid bacteria. The lactic acid bacteria H and lactic acid bacteria F of the purified number were inoculated into MRS medium respectively. In the reaction after 12 hours, measured at a wavelength of 600nm od by spectrophotometer. When the OD value is around 0.8, add 1ml 0.2 g/ml of nitrite. Using the method of hydrochloric acid, which is measured by the ultraviolet spectrophotometer in the wavelength of 538nm, the value of the nitrite in different reaction time, and the PH of the different response time period was measured by the PH of the laboratory.

Key words: Lactic acid bacteria; nitrite; The degradation; Naphthene diamine method

1 引言..1

1.1乳酸菌的简介1

1.2乳酸菌的功能1

1.2.1提供营养物质，促进机体生长1

1.2.2. 促进消化吸收.1

1.2.3. 改善胃肠道功能..2

1.2.4.改善免疫能力..2

1.2.5.抗菌.2

1.3乳酸菌的分离与筛选.2

1.3.1乳酸菌的分离.2

1.3.2乳酸菌的筛选..2

1.4乳酸菌的保存..2

1.5乳酸菌的鉴定..2

1.5.1菌落形态特征鉴定3

1.5.2生理生化特征鉴定.3

1.5.3分子生物学水平的鉴定3

1.6乳酸菌的活度检测.3

1.6.1平板涂布计数法（国标法）. 3

1.6.2紫外分光度法 .. .3

1.7亚硝酸盐中毒.. .. . .3

1.8亚硝酸盐致癌4

1.9亚硝酸盐用途4

1.9.1保色剂 .4

1.9.2提高食用肉制品的安全性..4

1.9.3 增进肉的风味和防腐剂的作用4

1.10亚硝酸盐的检测4

1.10.1亚硝酸盐的定性检测..4

1.10.2可见分光光度法..4

1.10.3紫外分光光度法5

1.10.4电位分析法5

1.10.5离子色谱法5

1.11降解亚硝酸盐的途径5

1.11.1 生物降解亚硝酸盐5

1.12 本课题的研究内容、目的及意义6

2实验材料与方法7

2.1实验材料.7

2.1.1实验试剂及样品信息7

2.1.2培养基.7

2.2主要仪器及设备7

2.3菌种.8

2.4实验方法..9

2.4.1乳酸菌提纯9

2.4.2乳酸菌的扩培9

2.4.3乳酸菌的甘油保存9

2.4.4亚硝酸盐含量的测定..10

2.5降解亚硝酸盐实验.. .11

2.5.1 0.2g/ml亚硝酸钠溶液配制.. .11

2.5.2 菌液盐酸萘乙二胺法测亚硝酸盐含量11

2.5.3 降解亚硝酸盐步骤11

2.6菌液PH测定. ..12

2.7乳酸菌生长曲线的绘制.12

3结果分析 ..13

3.1亚硝酸盐含量随着时间的变化13

3.2随着时间的增长，乳酸菌降解亚硝酸盐的情况16

3.3样液PH随时间的变化规律20

3.4乳酸菌生长曲线 .23

致谢 .. 24 亚硝酸盐污染生物处理工艺设计:http://www.youerw.com/shengwu/lunwen_80812.html