摘 要：目的：本实验研究8%、15%浓度的酒精对小鼠记忆的影响。方法：将30只小白鼠分为三组，一组为对照组，饲养期间每天喂食蒸馏水，另外两组为实验组，一组每天喂食8%的酒精，一组每天喂食15%的酒精，饲养5w后，对三组小鼠分别进行水迷宫测试，记录其找到平台的时间。结果：与对照组相比，实验组小鼠的学习、记忆能力均表现出下降的趋势(p＜0。05 或p＜0。01)，15%浓度的小鼠记忆损伤比8%严重。结论：酒精对小鼠的记忆有明显的损害作用，导致学习记忆能力下降。78696
毕业论文关键词：小鼠，酒精，学习记忆，水迷宫
Abstract:Objective: the effects of 8% and 15% concentration of alcohol on the memory of mice were studied in this experiment。 Methods: 30 mice were randomly pided into three groups, a group among them was fed daily with distilled water daily as control, and the other two groups were treated as the experimental group by the means of feeding them 8% alcohol and 15% alcohol separately, three groups of mice were conducted with water maze test, also the time which the mice found the platform was recorded。 Results: compared with the control group, the the mice’s ability of learning and memory in the experimental group showed a trend of decrease (P < 0。05 or P < 0。01), and the 15% concentration of the memory impairment was more serious than 8%。 Conclusion: alcohol had significant effects on the memory of mice, leading to the decline of the ability of learning and memory。
Keywords:mice,alcohol,learning and memory,water maze test
目 录
1 前言 4
2 材料与方法 4
2。1 材料 4
2。1。1 实验材料、试剂及配制 4
2。1。2 实验仪器、用品 4
2。2 方法 4
2。2。1 动物分组、标记 4
2。2。2 动物喂养 4
2。2。3 通道式水迷宫 5
2。3 统计学处理 5
3 结果分析与讨论 5
3。1 通道式水迷宫实验结果 5
3。2 通道式水迷宫实验分析 6
3。3 讨论 7
3。3。1 学习和记忆的神经生理学机制 7
3。3。3 学习记忆的神经干细胞假说 8
结论 10
参 考 文 献 11
致 谢 12
1 前言
酒精中毒已经发展成全球普遍的常见病症，酒精依赖、酒精滥用已是世界各国较为常见的社会问题。酒精滥用与高犯罪、高离婚率、高交通事故率有明显相关。据有关报导[1],全球职业人口中大约有11%的人酗酒,给社会和家庭造成了非常大的经济损失;同时,对人类的健康也造成巨大威胁，尤其是在欧洲，其发病率仅次于心脑血管疾病和肿瘤；我国随着经济发展水平的不断提高，酒精中毒也逐渐增多，现在临床上对酒精中毒引起的神经系统损害现象日益增长。
不同浓度酒精喂养后小鼠学习和记忆影响:http://www.youerw.com/shengwu/lunwen_90754.html