酵素中乳酸菌高密度培养及冷冻干燥工艺研究

摘要：本论文以植物乳杆菌作为菌种，通过工艺优化进行高密度培养，对获得的菌体进行冷冻干燥处理，并优化冻干工艺。首先以pH和活菌数为指标，通过单因素对乳酸菌发酵过程进行优化，最后通过响应面实验，得出最适培养温度为37℃，初始pH6。30，添加2%酵母抽提物、果糖、麦精，0。7%番茄汁以及接入1。1%乳酸菌培养24小时，活菌数为4。4×109CFU/mL。通过进行冷冻干燥的工艺优化，最终获得最适冻干保护剂配方：脱脂乳粉100g，乳糖100g，抗坏死血酸钠10g，谷氨酸钠10g。1:10比例与菌泥混合，得到的冻干菌粉活菌数为4。3×1011CFU/g。81291

毕业论文关键词：酵素；高密度培养，乳酸菌；活菌数；冷冻干燥

Study on high density culture of lactic acid bacteria in Biozyme and optimization of freeze-drying process

Abstract: In this paper, Lactobacillus plantarum was used as a strain, and high density culture was optimized by process optimization。 The obtained cells were freeze-dried and optimized。 Firstly, the fermentation process of lactic acid bacteria was optimized by single factor, and the optimum culture conditions were as follows: 37 ℃ and pH6。30 were used to extract 2% yeast extract。 , 0。7% tomato juice and 1。1% lactic acid bacteria were cultured for 24 hours, the viable count was 4。4 × 109CFU/mL。 The final freeze-dried protective agent formula was: degreased milk powder 100g, lactose 100g, sodium necroicide sodium 10g, sodium glutamate 10g 1L distilled water to dissolve and mix to 1:10 The ratio of bacteria and bacteria mixed, the freeze-dried bacteria powder viable count of 4。3 × 1011CFU/g。

Key words: Enzyme; high density culture, lactic acid bacteria; viable count; freeze drying

1 绪论 1

1。1酵素的概念、功能及其产品形式 1

1。1。1酵素的概念及功能 1

1。1。2酵素的制备 2

1。2制备酵素粉中的关键技术 3

1。2。1乳酸菌的高密度培养 3

1。2。2 乳酸菌的冷冻干燥 3

1。3本课题研究目的及意义 3

2 材料与方法 4

2。1 菌种、培养基与试剂以及配置方法 4

2。2 仪器与设备 5

2。3 实验方法 6

2。3。1菌体活化以及果汁的制备 6

2。3。2 pH计的校正及测定 6

2。3。3菌体密度的测定 6

2。4单因素实验 6

2。4。1最适培养时间 6

2。4。2最适pH 6

2。4。3最适碳源 7

2。4。4最适缓冲盐 7

2。4。5最适添加物 7

2。5 响应面的优化和验证 7

2。5。1 响应面方案设计 7

2。5。2 回归模型的建立及方差分析 8

2。6 冷冻干燥工艺优化 8

2。6。1冷冻干燥的操作步骤 8

2。6。2 冻干保护剂的单因素实验酵素中乳酸菌高密度培养及冷冻干燥工艺研究:http://www.youerw.com/shengwu/lunwen_94892.html