透明质酸基复合水凝胶的原位交联及性能
摘要本文设计和合成了一种可注射型复合水凝胶支架材料。该复合水凝胶由透明质酸和地塞米松接枝的壳聚糖交联而成。这种复合凝胶化通过水溶性壳聚糖分子中的氨基与氧化透明质酸分子中的醛基发生席夫碱反应形成，整个交联过程为共轭加成，因此无需添加其它化学交联剂，保证了该体系的细胞相容性。本文采用的水溶性壳聚糖为N-琥珀酰-壳聚糖，它进一步通过水溶性碳二亚胺催化，共价接枝了地塞米松。本文研究了该复合水凝胶支架材料在磷酸盐缓冲液（PBS）中的凝胶化时间、支架形态、溶胀、降解等体外性能。研究发现，与未接枝地塞米松的复合水凝胶相比，地塞米松接枝的水凝胶支架表现出更少的凝胶时间、更高的水分吸收和更快的降解速度。这种地塞米松接枝的水凝胶支架，有望更好地促进脂肪干细胞的粘附和增殖，可作为一种潜在的可注射细胞支架在脂肪组织工程中得到应用。67785
毕业设计说明书（论文）外文摘要
Title Crosslinking and Properties of Hyaluronic Acid Hybrid Hydrogel
Abstract
In this article, injectable hybrid hydrogels were designed and produced. This kind of hybrid hydrogels were produced by mixing crosslinked hyaluronic acid with dexamethasone grafted chitosan. The gelation is attributed to the Schiff-base reaction between amino groups of water soluble chitosan molecules and aldehyde groups of hyaluronic acid oxide. The whole procedure is conjugate addition; therefore it is without any other chemical crosslinking agent, which ensures the biocompatibility of the system. We use N-succinyl-chitosan as the water soluble chitosan, which is grafted with dexamethasone via water soluble carbodiimide catalysis. We study morphologies, swelling, weight loss, and compressive modulus of hybrid hydrogels in phosphate buffered saline in vitro gelation time in this article. Results demonstrated that compared to the hydrogel without dexa-methasone, the dexamethasone grafted hydrogel showed a slightly lower gelation time, higher water uptake and faster weight loss. The dexamethasone grafted hydrogel resulted in enhanced cell adhesion and proliferation as compared to the hydrogel without dexamethasone, which provide a potential opportunity for the dexamethasone grafted hybrid hydrogel as an injectable scaffold in adipose tissue engineering applications.
Keywords Biomaterials, Biodegradable, Gels, Tissue Engineering
目 录
1 前言 1
1.1 研究背景 1
1.2 支架材料 4
1.3 课题提出 6
2 实验部分 8
2.1 原料及试剂 8
2.2 醛透明质酸（AHA）的合成 8
2.3 N-琥珀酰-壳聚糖（SCS）的合成 8
2.4 地塞米松接枝壳聚糖（SCS-DEX）的合成 9
2.5 水凝胶的制备 9
2.6 水凝胶的表征 10
3 结果与讨论 11
3.1 水凝胶的形成 11
3.2 水凝胶的微结构 13
3.3 水凝胶的溶胀性质