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摘要：迷迭香在药品、食品等多个方面都有很好的应用前景。本文以迷迭香作为实验原料，采用超声波提取技术，对迷迭香有效成分进行提取，通过单因素实验分别考察了提取溶剂、提取时间以及料液比对迷迭香有效成分提取率的影响。并采用抑菌圈和最小抑菌浓度法考察迷迭香有效成分的抑菌效果。结果表明：迷迭香有效成分最佳提取条件是，常温条件下，用75%乙醇，物料比为1:28时超声提取25min。此时迷迭香提取物的提取率最高。所提取的迷迭香提取物其抑菌效果随着迷迭香提取物浓度的升高而增强。按照试验成果表明，6%浓度的迷迭香提取物迷迭香酸抑菌效果最好。34777
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毕业论文关键词：迷迭香；迷迭香酸；抑菌；提取率
Extraction of active constituents from rosemary and its antibacterial effect evaluation
Abstract: Rosemary's value has been proven in many aspects of drugs, food, etc.. There are many good prospects of application results in these areas. In this paper, I will use five concentrations ethyl alcohol in 15%, 35%, 55%, 75%, 95% as a solvent extraction process, the active ingredient of rosemary research, based on the extraction of the active ingredient in rosemary fans Rosemary acid, and inhibition zone method to evaluate its antimicrobial efficacy with the lowest concentration of extract of E. coli inhibition study its application in matrix cream and other cosmetics. The results show that: The best extraction condition of rosemary active ingredient was: under normal temperature condition, 1:28 when the ultrasonic extraction was 25min and 75% ethanol. The extraction rate of rosemary extract was the highest at that situation. The extracts of the rosemary extract were enhanced with the increase of the concentration of the extract. According to the results of the experiment, the antibacterial effect of the 6% concentration of rosemary extract is the best.
Rosemary have proved some application in cosmetics antibacterial, it also can be used as plant extracts added to cosmetics.
Key word: rosemary; rosemary acid; bacteriostat；extraction rate
目录
1 前言 1
1.1 迷迭香国内外研究现状 1
1.1.1 迷迭香有效成分 1
1.1.2 迷迭香酸的性质 1
1.2 迷迭香酸的研究方法 2
1.2.1 超声波辅助提取法提取迷迭香酸 2
1.2.2 超临界二氧化碳萃取法提取迷迭香酸 2
1.2.3 纤文素酶辅助提取法提取迷迭香酸 2
1.2.4 溶剂法提取迷迭香酸 3
1.2.5 常规热回流提取法提取迷迭香酸法 3
1.2.6 微波法提取迷迭香酸 3
1.2.7 pH控制匀浆法提取迷迭香酸 3
1.3 抑菌研究方法现状 4
1.3.1 纸片扩散法 4
1.3.2 稀释法 4
1.3.3 杯碟法杯 4
1.3.4 琼脂稀释法 4
1.4 本课题研究的目的和意义 5
2 实验原理与方法 5
2.1 实验原料与仪器设备 5
2.1.1 实验原料 5
2.1.2 实验仪器与设备 7
2.2 实验步骤 9
2.2.1 迷迭香粉末制备 9
2.2.2 迷迭香酸提取效率的探究 9
2.2.3 含迷迭香提取物乳液的配制 10
2.2.4 含迷迭香成分乳液性能的测试 12
2.3 迷迭香提取物抑菌效果的测定 12
2.3.1 配制培养基 12
2.3.2 菌种的准备 13
2.3.3 抑菌圈灭菌法 13
2.3.4 最小抑菌浓度法 14