聚谷氨酸/壳聚糖水凝胶的制备及性能表征
毕业论文关键词:γ-聚谷氨酸；壳聚糖；水凝胶；降解性
TPGA / chitosan hydrogel preparation and characterization
Abstract: In this experiment, respectively from the inoculation amount, water content, the concentration of sodium chloride, magnesium ion concentration, the concentration of potassium hydrogen phosphate two and a series of factors of single factor test, analyzed the factors of optimal value of medium optimization of solid fermentation of γ -PGA. The results showed that the inoculation amount, concentration of sodium chloride, potassium hydrogen phosphate two concentration was the main factor influencing the production of γ -PGA. The inoculation was 1.4ml, the concentration of sodium chloride was 0.78%, concentration of potassium hydrogen phosphate 0.003mmol/g two. Under these conditions, the yield of 0.1915g γ -PGA, than before optimization of gamma polyglutamic acid production increased by 6.5%. Then the chitosan / hydrogel preparation and characterization, results indicated that in 37 ℃ PGA / chitosan hydrogel has good performance and swelling, swelling in
the neutral condition was the best, the saturated swelling rate of 120%. equilibrium swelling of hydrogel in acidic conditions deswelling faster than the swelling rate of neutral under the conditions of. And good biodegradability, degradation and 37 ℃ with 1mg/ml papain 40 hour rate reached 58%.
Key words: γ -PGA；chitosan；hydrogels ；degradability
目录
1 引言 1
1.1 γ-聚谷氨酸 1
1.1.1 结构与性质 1
1.1.2 应用 1
1.2 壳聚糖 1
1.2.1 结构与性质 1
1.2.2 应用 2
1.3 水凝胶研究的意义 3
1.4 国内外研究现状概述 3
1.4.1 水凝胶的基本性质 3
1.4.2 水凝胶的应用[9] 4
1.4.3 水凝胶的研究前景[10] 5
1.4.4水凝胶的制备方法[11] 5
2 实验材料与方法 6
2.1 实验材料 6
2.1.1 实验试剂及药品 6
2.1.2 实验仪器 7
2.1.3培养基 7
2.2 实验方法 7
2.2.1 γ-聚谷氨酸的发酵制备 7
2.2.2 固体发酵γ-聚谷氨酸的优化 8
2.2.3 γ-PGA水凝胶的制备 9
2.2.4 聚谷氨酸/壳聚糖水凝胶的性能表征 10
3 结果与讨论 11
3.1 单因素试验结果 11
3.2聚谷氨酸/壳聚糖水凝胶的性能表征结果 12
3.2.1水凝胶的溶胀动力学曲线 12
3.2.2水凝胶的pH敏感性测试 13
3.2.3水凝胶的溶胀-消溶胀特性测试 14
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