近零磁场对白背飞虱生长发育和翅型分化的影响研究
毕业论文关键词：白背飞虱；近零磁场；生长发育；翅型分化；磁生物学效应；
Research on the effects of near zero magnetic field on growth and development and the differentiation of wing type in the white-back plant hopper
Abstract： White-back plant hopper, a kind of rice plant hopper, is one of the most important rice pests in Asia. Besides, the Earth's magnetic field as a ubiquitous non-biological factor, in addition to the role of animal behavior in the direction of its strength changes can also produce biological effects of biological. Based on insect ecology, this paper studied the magnetic and biological effects of near-zero magnetic field on the development of white-back plant hopper (Lepidoptera) from the aspects of growth and development and wing differentiation. The results show that the near-zero magnetic field significantly prolongs the egg period and the developmental period of nymphs. In the case of wing type differentiation, the ratio of short wing of white-back plant hopper was significantly higher than that of normal magnetic field. On the other hand, the time-dependent dynamic expression analysis of key genes JHAMT, FAMeT and JHEH and the key gene CYP307A1 of the echelon synthesis pathway were studied. The results showed that the near-zero magnetic field and the changes of juvenile hormone and eczema hormone titer are consistent, reflecting the near-zero magnetic field may affect the development of the white-back plant hopper nymphs by regulating the hormone signal pathway.
Key words: white-back plant hopper；Near-zero magnetic field（NZMF）；Magnetobiology
目录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 1
1. 材料与方法 3
1．1 供试材料 3
1．2 NZMF发生装置的建立 3
1．3 主要试剂耗材及仪器设备 4
1．3．1 主要试剂耗材 4
1．3．2 仪器设备 4
1．4 白背飞虱在近零磁场中的暴露实验 4
1．5 白背飞虱各项研究测定方法 5
1．5．1 白背飞虱卵期的测定 5
1．5．2 白背飞虱若虫历期的测定 5
1．5．3 白背飞虱成虫翅型分化比测定 5
1．6 SYBR Green荧光定量PCR（qRT-PCR）分析 5
1．6．1 引物设计、合成与筛选 5
1．6．2 SYBR Green法实时荧光定量PCR 6
1．6．3 相对表达量的计算 7
1.7 数据处理与分析 7
2. 结果与分析 7
2．1 NZMF对白背飞虱卵期发育的影响 7