基于磁珠分离技术的氯噻啉上转换荧光免疫分析方法的研究
毕业论文关键词：氯噻啉；免疫磁珠分离；荧光纳米材料；上转换荧光免疫分析
The Development of Upconversion Fluorescence Immunoassay Based on Magnetic Beads
Abstract: Imidaclothiz is a kind of new neonicotinoid systemic insecticide acting on Nicotinic acetylcholine receptor.It has the advantages of high efficiency, low toxicity, broad spectrum. With the increasing of the use, the study on the methods of detecting residues in agricultural products and environment has been carried out extensively. The development of immunoassay of pesticide residues detection is rapid, the application is also gradually widely. It is sensitive, rapid, specific, large capacity, safe and reliable. Immunomagnetic bead isolation technology is a new immunoassay technology which combines the high specificity of the immunological reaction with the enrichment characteristics of magnetic beads. Assembled together to form composite nanomaterials, Fe3O4 magnetic nanoparticles and upconversion fluorescent nanomaterials play their respective advantages in separation and enrichment effect and optical characteristics. In the study, composite nanomaterials of Fe3O4 magnetic nanoparticles and upconversion fluorescent nanomaterials are synthesized with magnetic separation technology. The upconversion fluorescence immunoassay method is established and applied to analysis of the residues of Imidaclothiz in produce and environmental samples.
Key words: Imidaclothiz; Immunomagnetic separation; Fluorescent nanomaterials; Upconversion Fluorescence Immunoassay
目 录
引言 2
1 材料与方法 3
1.1 材料 3
1.1.1 主要试剂 3
1.1.2 主要仪器 3
1.2 方法 4
1.2.1 上转换纳米材料（UCNPs）合成 4
1.2.2 上转换纳米材料（UCNPs）氨基功能化 4
1.2.3 氨基功能化Fe3O4 磁性纳米颗粒（MNPs）合成 4
1.2.4 纳米材料与氯噻啉人工抗体、抗原偶联 4
1.2.5 基于UCNPs和MNPs免疫分析 4
1.2.6 免疫分析工作条件优化 5
1.2.7 选择性评价（交叉反应） 5
1.2.8 添加回收实验 5
1.2.9 准确性验证 5
2 结果与分析 6
2.1 磁性纳米颗粒表征 6
2.2 免疫分析工作条件优化 7
2.3 灵敏度分析 8