摘 要本次实验研究并分析了温度（16℃、23℃、30℃）本次研究比较观察了不同温度包含自然发生于原产黑龙江流域的平均温度16℃、，及仔鱼开始拒食的最高温度30℃及中间温度23℃。和光周期（0h、12h、24h）光照范围来自无光照与全天光照对乌苏里拟鲿卵黄体利用率的影响，采用了中心复合设计(CCD)和响应曲面法（RSM）进行分析。94039

1。试验结果表明温度的一次效应、二次效应对乌苏里拟鲿卵黄体利用率具有极显著影响（P<0。01），仔鱼卵黄体利用率随温度增高呈先升高后降低的趋势。

2。光周期的二次效应对乌苏里拟鲿卵黄体利用率具有极显著影响（P<0。01），而一次效应对乌苏里拟鲿卵黄体利用率不具显著影响（P>0。05）仔鱼卵黄体利用率随光周期增长呈先升高后降低的趋势。

3。温度、光周期间的互作效应对乌苏里拟鲿卵黄体利用率无显著影响(P>0。05)。且温度效应大于光周期效应。建立了温度、光周期对乌苏里拟鲿卵黄体利用率的模型，从而为批量养殖提供理论参考。

4。通过对2个指标同时进行优化，得出最优组合温度为28℃，光周期为18h，模型可信度为，说明模型具有较高的可信度，可为乌苏里拟鲿生产提供理论指导。

温度、光周期对乌苏里拟鲿卵黄体利用率的实际二次多项回归方程为：

Y=-183。9892+18。67178×T+1。77756 ×P+9。23E-03×T×P-0。32664×T2-0。067398×P2

回归方程决定系数为0。9865、校正系数为0。9729，说明所拟的二次方程合适。

毕业论文关键词：乌苏里拟鲿；响应曲面；温度；光周期

ABSTRACT The experimental study and analysis of the temperature (16 ℃, 23 ℃, 30 ℃) in this study were observed at different temperatures containing naturally occurring in the Heilongjiang Province, the average temperature of 16 ℃, and larvae began to eat the maximum temperature of 30 ℃ And an intermediate temperature of 23 ° C。 And photoperiod (0h, 12h, 24h) were used to analyze the effects of no light and all-day illumination on the utilization of yolk inUusuand were analyzed by center composite design (CCD) and response surface method (RSM)。

1。 The results showed that the effect of temperature and secondary effect had a significant effect on the utilization of yolk of Uusu(P <0。01)。 The utilization rate of egg yolk in larvae increased first and then decreased with the increase of temperature。

2。 The effect of primary effect on the utilization of yolk ofUusuya was not significantly affected by the secondary effect of photoperiod (P <0。05) The utilization rate of the corpus luteum increased first and then decreased with the increase of photoperiod。

3。The interaction between temperature and light weeks had no significant effect on the utilization rate of yolk in Usuli(P> 0。05)。 And the temperature effect is greater than the photoperiod effect。 The model of temperature and photoperiod on the utilization rate of yolk of Uusuya was established, which provided a theoretical reference for batch culture。

4。Through the optimization of the two indexes at the same time, the optimal combination temperature is 28 ℃, the photoperiod is 18h, the model reliability is that the model has high reliability, which can be used for the production of WusuliProvide theoretical guidance。 The actual quadratic regression equation of temperature and photoperiod on the utilization rate of corpus luteum in Wusuliwas:

Y = -183。9892 + 18。67178 × T + 1。77756 × P + 9。23E-03 × T × P-0。32664 × T2-0。067398 × P2

The regression equation is 0。9865 and the correction coefficient is 0。9729, indicating that the proposed quadratic equation is appropriate。