氯化钾提高玉米茎腐病的蛋白质组学分析
毕业论文关键词：玉米茎腐病，KCl，蛋白质组学，基因本体论
Proteomics Analysis of Potassium Chloride to Improve Maize Resistance to Stalk Rot
Abstract:Corn stalk rot,one of the corn important diseases,affects corn production seriously.KCl takes an active work in resistant responses to diseases of corn.The experiment was pided into three groups,Jidan 180 infected by Fursarium graminearum,co-treated infected by Fursarium graminearum and feeded by KCl,and planted normally.The analysis for total proteins of Corn was conducted by proteomic method from the point of GO and KEGG.The results indicate that two hundred and thirty eight protein spots exist differentially expressed and these proteins are involved in many typed of processes.From the point of view of gene ontology, it is speculated that the regulatory proteins of KCl to improve the resistance to stem rot of maize are mainly located in the cytoplasm, which can be realized by regulating the redox process. From the analysis of the KEGG pathway annotation angle, KCl enhanced the resistance of corn stalk rot related protein mainly involved in the metabolic regulation of the synthesis of cyano amino acid metabolism, limonene and pinene degradation pathway and phenylpropanol biosynthesis pathway of these three types of resistance to achieve.
Key words:corn stalk rot,potassium chloride,proteomics ,GO annotations
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1试验材料 2
1.2实验方法 3
1.3全蛋白的质谱鉴定及差异分析4
1.4差异蛋白的功能查询4
2结果与分析4
2.1差异表达蛋白鉴定分析4 2.2差异蛋白生物功能分析5 2.3差异蛋白的KEGG分析14 3讨论 16
3.1差异表达蛋白在细胞内的定位与抗性的关系16 3.2差异表达蛋白所行使的分子功能与抗性的关系17
3.3差异表达蛋白所参与的生物过程与抗性的关系17
3.4差异表达蛋白所参与的代谢通路与抗性的关系18
致谢18
参考文献18
氯化钾提高玉米茎腐病的蛋白质组学分析
引言
玉米茎腐病，又称玉米青枯病、茎基腐病等，是一种在世界上广泛分布并发生的一种土传病害，主要由腐霉菌、炭疽菌、镰刀菌侵染引起，在中国、日本、加拿大、美国、印度等至少23个国家发生[1]。1980年起，该病在中国各玉米产区相继发生，一般年份产量损失在 20%~30%左右，局部地区产量损失达 50% 以上，严重时甚至绝收[2]。经过国家“八五”和“九五”攻关研究，明确了其病原菌的优势种组成及该病害的栽培防病关键措施，但该病害的发病率仍然较高。近年，我国随着种植结构的调整和耕作栽培制度的变革，种植玉米品种多样性日益明显，选育并推广了一系列诸如高淀粉、高油、高赖氨酸，耐密紧凑型的高产杂交种。但是，由于这些品种遗传基础狭窄，缺少抗性基因，且相应的综合防治措施没有及时跟上去，再加之近年来，保护性耕作、秸秆还田及气候的影响，玉米茎腐病又有加重的趋势，从而导致玉米茎腐病从次要病害上升为主要病害[3,4]。