典型环境污染物强力霉素和草甘膦的水生植物毒性研究
毕业论文关键词：强力霉素；草甘膦；铜绿微囊藻；绿色微囊藻；氧化损伤
Aquatic Toxicity of Two Typical Environmental Pollutants
Abstract: Doxycycline hydrochloride and glyphosate have been used extensively worldwide. The residues of them can be discharged into water system through different exposure routes such as raining, surface runoff and infiltration, these two pollutants have high detection rate at environmentally relevant concentrations. Water blooms caused by cyanobacteria in aquatic system are major global environmental issues in recent years. However, the physiological effects on cyanobacteria caused by these two typical environmental pollutants are insufficient. In the present study, Microcystis aeruginos and Microcystis viridis were selected as non-target organisms to explore the ecotoxicity of doxycycline hydrochloride and glyphosate. The results showed that these two typical environmental pollutants could inhibit the growth of Microcystis aeruginos and Microcystis viridis under laboratory conditions and they could also induce oxidative damage. Glyphosate could stimulate the protein and chlorophyll a content of Microcystis aeruginos and Microcystis viridis. At the same time, glyphosate could result in early apoptosis of cells and the cellular structure has been changed. The results are useful for environmental assessment of these two typical environmental pollutants. Besides, it is also helpful for guiding the application of doxycycline hydrochloride and glyphosate in agricultural settings.
Key Words: doxycycline hydrochloride；glyphosate；Microcystis aeruginos；Microcystis viridis；oxidative damage
目录
1引言 1
1.1两种典型污染物的污染现状 1
1.1.1抗生素 1
1.1.2除草剂 1
1.2两种典型污染物的生态毒性 2
1.2.1强力霉素 2
1.2.2 草甘膦 3
1.3两种微囊藻概述 4
1.4 课题研究的意义与内容 5
2实验条件及依据 7
2.1实验用品 7
2.1.1实验仪器 7
2.1.2实验材料 7
2.1.3实验药品 8
2.2实验前期准备 9
2.2.1培养液的配置 9
2.2.2高压蒸汽灭菌 9
2.2.3微囊藻的接种 10
2.2.4微囊藻的培养 10
2.2.5强力霉素储备液的配制 10
2.2.6草甘膦储备液的配制 10
2.3实验方案论证 10
3两种污染物对两种藻的生长效应研究 12
3.1藻细胞数与吸光度的关系 12