长江中下游地区麦田禾本科杂草对精噁唑禾草灵抗性检测
毕业论文关键词：精噁唑禾草灵；杂草；抗药性；整株生物测定；抗性监测
Resistance detection of gramineous weeds in wheat fields to fenoxaprop in the middle and lower reaches of the Yangtze River
Abstract: In this paper, the gramineous weeds in wheat fields in Jiangsu, Anhui and Shanghai in the middle and lower reaches of the Yangtze River，including Alopcurus aequalis Alopecurus japonicus Beckmannia syzigachne, were selected and the their susceptibility to ACCase inhibitor herbicide fenoxaprop was studied to understand the occurrence and distribution of resistant gramineous weeds in wheat fields. The results were as follows: Among the 26 grass weed populations, 18 populations showed resistance to fenoxaprop with the proportion of resistance of 69.23%, in which most of the resistant populations were collected from Yangzhou , Changzhou, Lianyungang, Huai'an, Shanghai and other areas. The proportion of resistance occurrence reached 66.7% in Alopecurus aequalis reached and 50.00% in Alopecurus japonicus, while in Beckmannia syzigachne , it was 78.57%, which was higher than the other two species.
Key words :fenoxaprop ；weeds；Drug resistance ；whole-plant bioassay ； Resistance detection
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法1
1.1供试杂草种子1
1.2供试药剂 3
2 试验方法与数据处理3
2.1 试验方法 3
2.2数据处理3
2.3抗药性判断准 3
3 结果与分析3
3.1 日本看麦娘对精噁唑禾草灵的敏感性检测3
3.2 看麦娘对精噁唑禾草灵的敏感性 4
3.3 菵草对精噁唑禾草灵的敏感性4
3.4 长江中下游地区抗精噁唑禾草灵禾本科杂草地区分布5
4 全文讨论6
致谢7
参考文献8
长江中下游地区麦田禾本科杂草对精噁唑禾草灵抗性检测
日本看麦娘、菵草和看麦娘、是长江中下游地区小麦田主要发生的禾本科杂草。ACCase 抑制剂类除草剂精噁唑禾草灵是小麦田防除禾本科的常用除草剂。除草剂的长期连续单一使用可导致杂草对除草剂产生抗药性。迄今为止，已有报道看麦娘、日本看麦娘和菵草对精噁唑禾草灵的抗药性，但有关报道侧重于抗药性机理的研究，并未有文献资料系统报道抗药杂草的发生、分布和抗性水平等情况。因此，本研究采用整株测定法[1]，检测长江中下游地区麦田禾本科杂草看麦娘、日本看麦娘和菵草对精噁唑禾草灵的敏感性水平，了解该地区抗药性禾本科杂草的发生分布及抗药性水平,从而为生产上延缓或控制杂草对重要除草剂的抗药性提供理论依据和实践指导[2]，对确保小麦安全生产具有重要意义。
1 材料与方法
1.1 供试杂草种子
依据2013到2015年抗性检测实验结果选择有代表性的抗性杂草发生田块采集杂草种子，在长江中下游地区的江苏、安徽及上海用药后仍有杂草发生的小麦田采集疑似抗性的看麦娘、日本看麦娘、菵草。以江苏农科院采集的未用药小麦田禾本科杂草种群作为敏感种群。采集的杂草种子自然条件下晾干后放置于纸袋中室温条件下储存备用。采集信息及数量汇总见表1-1到表1-4。