金黄色葡萄球菌急性感染对大鼠子宫损伤及功能的影响
毕业论文关键词：金黄色葡萄球菌；炎症因子；Toll样受体；紧密连接；大鼠
Effects of acute Staphylococcus aureus infection on uterine injury and function in rats
Abstract： The purpose of this study is to investigate the effects of acute staphylococcus aureus infection on uterus through the rat model,including the inflammation of uterus and damage to the endometrium. Surgical infection after 24h, uterine necropsy showed infected group rats with bilateral uterine swelling, vascular congestionn; fluorescence quantitative PCR results revealed that TLR2, Myd88, NF-kB gene expression in uterine tissues of infected group was up-regulated, and the mRNA expression of inflammatory cytokine IL-1, IL-6, IL-8, TNF- alpha increased significantly; ELISA results showed that the concentration of inflammatory factors in peripheral blood of infected group were all significantly higher than those in the saline control group, indicating the occurrence of uterine inflammation after infection of Staphylococcus aureus. The expression of tight junction related proteins occludin and claudin4 mRNA increased, while the mRNA expression of ZO-1 and MUC1 decreased, indicating the endometrial damage and decreased barrier function.
Key words: Staphylococcus aureus；Inflammatory factors；Toll-like receptor；Tight junction；Rat
目 录
摘要3
关键词3
Abstract3
Key words3
引言3
1材料与方法4
1.1实验材料与试剂4
1.1.1实验动物4
1.1.2菌株4
1.1.3药品及试剂4
1.2实验仪器4
1.3实验方法4
1.3.1实验动物分组4
1.3.2菌株复壮与菌液稀释4
1.3. 3手术法感染菌液4
1.3. 4子宫剖检与样本采集5
2结果与分析 5
2.1子宫剖检结果5
2.2组织病理学检查结果6
2. 3 TLR2/Myd88/NF-KB信号通路上的基因表达6
2.4子宫组织中各细胞因子的mRNA表达量7
2. 5 外周血中炎症因子检测结果7
2.6紧密连接相关蛋白mRNA表达量7
3讨论 8
3.1大鼠子宫内膜炎模型的构建8
3.2急性感染造成大鼠子宫内膜损伤继而影响其生理功能8
致谢9
参考文献9
金黄色葡萄球菌急性感染对大鼠子宫损伤及功能的影响
子宫微生物感染影响着半数的产后奶牛，15%~20%的奶牛在产后持续三周以上表现明显的临床症状（临床型子宫内膜炎），而30%的奶牛则患有无临床症状的子宫慢性炎症（亚临床型子宫内膜炎）[1]。因为奶牛产后几天内产道保持开放，外界细菌很容易进入宫腔，虽然很多牛在产后前5周内会自主清除细菌，仍然有10%~17%的奶牛子宫腔被持续感染[2]。当子宫局部或全身免疫力下降时，病原菌就会在子宫内大量繁殖，造成感染发病，致使奶牛发情、排卵异常，并且扰乱产后卵巢优势卵泡的生长和发育，阻碍胚胎着床，导致母牛繁殖性能与产奶性能一齐下降，甚至青年母牛不孕[3]，此外，增加的人力、饲料、用药成本及治疗期间奶源的废弃都造成了极大的经济损失，早在1986年，就有学者估算，每个患病牛在泌乳期的总损失约为106美元[4]，因此，生殖道微生物疾病是奶牛上最为普遍也最具经济重要性的疾病[1]。