大豆品种对大豆花叶病毒的抗病鉴定和标记辅助选择
毕业论文关键词：大豆花叶病毒；标记辅助选择；抗病鉴定；SSR标记
Identification of Resistance and Marker Assisted Selection of Soybean Mosaic Virus Resistance Gene Rsc-3 and Rsc7 in Soybean
Abstract：This study selected 268 soybean varieties with different types of soybean germplasm resources from the Agricultural University National Center for Soybean Improvement. These soybean varieties were inoculated with the soybean mosaic virus (SMV) strains SC-3 and SC-7 for identification of their resistance. Therefore, we can further understand the level of China soybean breeding. Also, SSR markers linked to SMV resistance genes Rsc-3 and Rsc-7 in Kefeng No.1 and Qihuang No.1 were used for Marker assisted selection (MAS) in order to find resistant germplasm resources. The results showed that among the 268 varieties, 47.58% are resistant to SC-3 and 45.35% are resistant to SC-7(including HR and R). Middle resistance levels (MR and MS) account for SC-3 and SC-7 are 47.58% and 29.00%, respectively. The proportion of S and HS were very low, especially HS which was even less than 6%. The proportion of SC3 resistant varieties was higher than that of varieties resistant to SC7. The MAS efficiency of SSR markers Satt558, BARCSOYSSR_02_0610, Satt226, Satt114 and BARCSOYSSR_13_1136 was evaluated in the soybean varieties. 301、314、293、182 and 229 varieties were identified for the same characteristic bands of corresponding parents. According to different SSR markers, 160 (53.16%), 163 (51.91%), 149 (50.85%), 92(50.55%) and 97(42.36%) are correct for resistant situation of SC-3; 160(53.16%), 172(54.78%), 138 (47.10%), 89 (48.90%) and 103 (44.98%) are correct for the resistant of SC-7. Therefore, the SSR markers can be used effectively in selecting for resistance genes Rsc-3 and Rsc-7 instead of inoculation identification for some situations.
Key words: Soybean; Soybean mosaic virus；Gene mapping; Marker assisted selection
目 录
摘要3
关键词3
Abstract3
Key words3
引言（或绪论）4
1材料与方法4
1.1材料 4
1.1.1植物材料 4
1.1.2病毒株系 4
1.1.3 SSR引物选择合成5
1.2方法 5
1.2.1田间试验及温室接种鉴定5
1.2.2抗性分级与评价5
1.2.3 DNA的提取6
1.2.4 PCR扩增反应及产物的电泳检测6
1.2.5 分子标记带型分析6
2 结果与分析7
2.1 大豆对SMV株系SC3和SC7的抗性分析7
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