连云港盐田嗜盐古菌多样性研究
毕业论文关键词：灌南盐田；嗜盐古菌；16S rRNA基因；454高通量测序；多样性研究
Analysis of Archaea Diversity in Lianyungang GuanNan Saltern
Abstract: Acquisition Lianyungang Guannan salt brine, carries on the analysis using the 454 high-throughput sequencing of the archaea persity. Collected the archaea 16S rRNA gene as template for PCR amplification and the amplification of archaea sequences were sequenced,then received a total of 3542 optimization sequence and 535 OTUs. All the archaeas are classified into Euryarchaeota，Halobacteria, Halobacteriales, 99.86% is classified into Halobacteriaceae on the level of classification, only 0.14% rest is not classification to the classification level. At genus levels, the archaeas were classified into 15 genus, of which Halorubrum,Halonotius and Haloquadratum were dominant bacteria, accounting for total archaea sample number 53.63%, 22.25% and 10.29%. 7.68% sequence in the previous study were not be classified to species or had not been found, indicating that in Lianyungang Guannan saltern, there might be new species of Archaea; in the remaining has been classified to the species of archaea, 62.31% is unable obtained through the method of pure culture. However,using 454 high-throughput sequencing method measured most of archaea sequences in Guannan brine. Through the study of archaea persity in Lianyungang Guannan saltern, provide a theoretical basis for the Archaea resources development, but also provide a theoretical guidance to enrich and protect the resources of archaea species.
Keywords: Guannan saltern; archaea; 16S rRNA gene; 454 high-throughput sequencing; persity research
目 录
引言 1
1 研究材料与方法 2
1.1实验材料 2
1.1.1采样地点描述 2
1.1.2水样采集 2
1.1.3主要实验仪器与试剂 3
1.2实验流程方法 3
1.2.1卤水样品总基因组(genome)的提取 3
1.2.2卤水样品总基因组(genome)电泳分析 3
1.2.3PCR扩增16S rRNA基因 3
1.2.4 电泳分析PCR扩增产物 4
1.2.5 PCR扩增产物的胶回收 4
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