呼伦贝尔高原盐湖嗜盐细菌多样性分析
毕业论文关键词：极端嗜盐菌；微生物多样性；16S rRNA基因法；454高通量测序；呼伦贝尔高原盐湖
Analysis of Halophilic Bacteria Diversity in Hulun Buir Plateau Salt Lake，China
Abstract: The experimental study is based on 16S rRNA genes，and use the PCR technology to obtain the DNAs from Hulun Buir Plateau Salt Lake, then through 454 pyrosequencing test the sequencing of samples. Among of the results,take Hailar Salt Lake on behalf of the Hulun Buir Plateau Salt Lake.On the earth, from the sample, obtained 9903 valid reads, 8320 trimmed reads., and 1325 operational taxonomic units (OTUs). The bacterial community in the sample was dominated into 13 phylums ,in the phylums，The main categories is Proteobacteria(Proteobacteria);
the second is Bacteroidetes (Bacteroidetes). In each level has a large number of sequence belongs to the sequence of unknown sequences or not to be classified. Research the halophilic bacteria persity in the Hulun Buir Plateau Saline Lake can complement the plateau microbial species of salt lake resources of our country. On the other hand,the method to research the species of Hulun Buir Salt Lake can be good case for study of microbial species later。
Keywords: 16S rRNA gene；HaloBacteria; microbiology persity; 454 pyrosequencing; Hulun Buir Plateau Salt Lake
目录
引言 1
1材料与方法 2
1.1研究材料 2
1.1.1采样地点 2
1.1.2 采样地点描述 2
1.1.3 实验研究中的样品采集 2
1.2 研究方法 3
1.2.1提取样品的宏基因组DNA 3
1.2.2宏基因组DNA完整性的检测 3
1.2.3 16S rRNA 基因的PCR扩增 3
1.2.4 PCR产物胶回收 4
1.2.5 产物荧光定量 4
1.2.6 对定量后的样品进行454测序以及对相关数据进行处理 4
1.3 对测序所得到的数据的分析 4
1.3.1 统计实验获得的有效序列数据 4
1.3.2 统计去杂处理之后的优化序列数据 5
1.3.3 对OTU分类单元的分析 5
2结果与分析 5
2.1 对经测序所得数据的处理和分析 5
2.2 嗜盐细菌多样性指数与丰度分析 5
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