茄子WRKY转录因子家族基因的鉴定与系统进化分析
毕业论文关键字：WRKY；茄子；WRKY家族；基因；进化
Molecular Characterization and Evolutionary Analysis of the WRKY transcription factor family genes in Eggplant
Abstract：WRKY transcription factor is one of the top ten transcription factor in the higher plants in regulating plant growth and art plays an important role. About eggplant WRKYTF family, we master data were rare. The eggplant complete genome sequencing allow us to the analysis of the system in the species WRKY genes. 74 all WRKY genes was noted and classification in seven little group. Evolution and the linear analysis shows that the eggplant all WRKY gene and arabidopsis thaliana, originated from a common ancestor. Genetic structure analysis showed that: eggplant WRKY transcription factor gene exon number variation range is 2-7, most of the WRKY transcription factors have 2-3 exons Protein. Sequence analysis revealed that the eggplant WRKY transcription factors have 18 conservative Motif, in addition to the Group I and Group II, core conservative Motif area (Motif 1-2) on the remaining five exist, in the Group and its organization mode is the same, illustrate the core conservative Motif area is common to the WRKY transcription factors. Using the sliding window method, found that eight of homologous genes, 7 to by negative selection function, only 1 on by choosing different stages. gene expression level comparative analysis shows that in eggplant WRKY homologous gene, gene expression differences do exist, further confirmed the dual WRKY gene functional differences.
Key words: WRKY transcription factor, Eggplant, WRKY family Gene, Evolution
目 录
摘 要 3
引言 4
1.数据与方法 5
1.1茄子WRKY基因检索与序列下载 5
1.2茄子WRKY基因序列比对与系统发生关系分析 5
1.3茄子WRKY蛋白保守基序组成模式分析 5
1.4茄子WRKY蛋白的选择压力检测 5
2.结果与分析 6
2.1茄子WRKY家族基因的鉴定 6
2.2茄子WRKY家族基因的系统进化关系 9
2.3茄子WRKY家族基因的结构特征 10
2.4茄子WRKY家族蛋白保守基序的组成模式 12
2.5基于滑动窗口检测同源基因对的选择压力 14
参考文献 16
附录 17
致谢 20
茄子WRKY转录因子家族基因的鉴定与系统进化分析引言
转录因子（transcription factor, TF）也称反式作用因子，是指那些具有同真核生物启动子特定DNA序列结合活性的蛋白质分子[1]。植物体内存在大量的转录因子，其主要功能是激活或抑制基因的转录效应。 WRKY是近年来新发现的植物特有新型锌指型转录调控因子， 因在其N-端含有由WRKYGQK组成的高度保守的7个氨基酸序列而得名。第一个WRKY转录因子是由Ishiguro等[1]于1994年从白薯中克隆得到， 随后在野燕麦、荷兰芹、拟南芥、马铃薯、白英、烟草和水稻中相继发现[2]。近年来，在拟南芥(Arabidopsis thaliana)、玉米(Zea mays)和矮牵牛(Petumia hybrid)等主要的模式植物中已经研究的很清楚[3]。已有很多证据表明：在果实成熟、种子表皮毛发育、蔗糖信号传导及赤霉素信号传导等过程中均发现了WRKY基因的表达[4-6]。 目前在拟南芥发现了74个WRKY成员，而水稻中则发现了105个完整的WRKY成员。研究发现，WRKY家族成员包含至少一个WRKY域，大约60个氨基酸组成的高度保守的标记锌指结构C2H2(C-X4-5-C-X22-23-H-X-H)或C2HC(C-X7-C-X23-H-X-C)。WRKY结构域及其专一结合的(T)(T)TGAC (C/T)序列(又称W-box)更多地存在于与抗病、损伤、衰老相关基因和水杨酸诱导基因的上游调控区域[7]。在茄科物种内已经有番茄、优尔椒、土豆和烟草等物种被研究[8]。