HPLC灵芝孢子粉中麦角甾醇的定量分析
毕业论文关键词：灵芝孢子粉；麦角甾醇；HPLC；方法学考察
The ergosterol content in ganoderma lucidum spore power
Abstract: In the past few years, the edible fungi are popular with many people because of its unique flavor ;nutritional composition and pharmacological action. Ganoderma play a very important part among it, ganoderma attracts many people’s attention because of its nearly 2000 years of medicinal value. It is a good herb with tonic effect. The recent research shows that ganoderma contain a lot of sterols especially ergosterol has a large proportion of it, and ergosterol also has important medical value. This article takes ganoderma lucidum spore powder as materials, after a series of research and analysis ,establishes HPLC analysis method to analyze the content of erogoster in ganoderma lucidum spore powder, and selects Aglient ZORBAX SB-Aq（4.6mm×250mm，5μm）as chromatographic column. The mobile phase consists of - (0.1%) acetic acid aqueous solution, with a flow rate of 1.0ml/min. The wavelength of determination is 282nm, and the column temperature is 30℃ . Methodological study shows that the method has good reproducibility, high precision and good reproducibility. So the method is suitable for the qualitative analysis of ergosterol in lucidum spore powder. The result shows the ergosterol content in broken lucidum spore powder of different breaking time, different storage temperature ,different sampling periods and different areas.
Key Words: Ganoderma lucidum spore powder; Ergosterol; HPLC; Methodological investigation
目录
1 前言 1
1.1 灵芝孢子粉 1
1.2 灵芝孢子粉的药用价值 1
1.2.1肿瘤的辅助治疗 1
1.2.2 降血脂的功效 2
1.2.3 抗神经衰弱作用 2
1.2.4 降血糖作用 2
1.3 麦角甾醇 3
1.4麦角甾醇研究现状 3
1.4.1麦角甾醇与文生素D2的关系 3
1.4.2麦角甾醇与真菌微生物量的关系 3
1.4.3麦角甾醇的生理作用 4
1.5HPLC 4
1.6课题研究意义与目的 5
2 实验内容 6
2.1实验材料、试剂与仪器 6
2.1.1实验材料 6
2.1.2 实验试剂与仪器 6
2.2 实验方法 6
2.2.1高效液相色谱条件的确定 7
2.2.2标准品溶液的制备 7
2.2.3供试样品溶液的制备 7
2.2.4麦角甾醇检测波长的确定 7
2.2.5方法学验证 8
2.2.5.1标准曲线的绘制 8
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