多肉花卉落地生根离体培养
毕业论文关键词： 落地生根；离体培养；植物生长调节剂
Tissue Culture of Kalanchoe daigremontiana
Abstract: Putting down roots (Kalanchoe daigremontiana) is a perennial succulent herb. It is main representative plants belonging to Crassulaceae (Crassulaceae) air plant genus (Bryophllum Salisb) .This experiment to take root tender leaves and adventitious buds as explants, with different concentration ratio of plant growth regulator of culture medium were callus induced adventitious buds induced rooting culture and acclimatization established tissue culture and rapid propagation of the reproductive system of roots. Was observed after inoculation Kalanchoe daigremontiana a leaves and stems of the growth status and record the rooting rate, the rate of pollution, and the callus differentiation rate. For the leaves of the callus, the growth status of the callus was mainly observed and the callus induction was observed.Observe its differentiated cells (ie, formation process of root and bud ). Comparative experiments can be drawn: in 6 different plant hormone concentration ratio of the medium, the most suitable for callus induction of 1 / 2MS medium for Group 1: 6BA0.2mg / L + NAA0.2mg / L ( That 6BA: NAA = 1: 1); the most suitable 1 / 2MS media to induce adventitious buds for group 4: 6BA1.0mg / L + NAA0.2mg / L (ie 6BA: NAA = 5: 1), adventitious bud induction rate of 93.3%; the most suitable for inducing adventitious root 1 / 2MS medium for the group E：6BA0.1mg / L + NAA1.0mg / L (ie 6BA: NAA = 1:10), rooting rate was 86.7%.
Keywords: Kalanchoe daigremontiana；Tissue culture；Plant growth regulators
目录
1 绪论 .1
1.1 落地生根属简介 ......1
1.2 落地生根的形态特征 .2
1.3 落地生根的生长特点...2
1.4 落地生根植物研究概况和存在的问题...3
1.5 课题研究目的和意义...3
2 实验材料与方法 ..3
2.1 实验材料..4
2.2 实验方法..4
2.2.1 无菌材料的获得............................4
2.2.2 愈伤组织的诱导............................4
2.2.3 芽分化及再生.............................4
2.2.4 愈伤组织诱导生根...............................4
2.2.5 实验测定项目.......................................4
3 结果与分析.5
3.1 不同灭菌方法对落地生根外植体的灭菌效果.....5
3.2 不同激素配比对落地生根外植体愈伤组织形成的影响..........7
3.3 不同激素配比对落地生根外植体不定芽形成的影响............10
3.4 不同激素配比对落地生根不定根诱导的效果..........12