细菌群体感应lux启动子的调控机制研究
毕业论文关键词： 群体感应；酶标仪；Plux启动子；crp序列；luxbox序列
Study on the mechanism of promoter regulation of bacterial quorum sensing
Abstract: Quorum sensing phenomenon was first observed in Vibrio fischeri, in the Vibrio fischeri, quorum sensing regulates the luminescence phenomena from bacteria themselves .There is no information exchange between bacteria? The similar inference and hypothesis was proposed by scientists ,and now ,it is confirmed that there is the information exchange between bacteria . But at the very beginning, people thought it could not be confirmed. What is the connection between bacteria?It's all worth exploring for human beings to communicate the information between the bacteria which are beneficial or harmful.This experiment was carried out in order to study the mechanism of initiation of bacterial quorum sensing. The strains used in this experiment (St316, St336, St346, St319) are all derived from our own laboratory. In the experiment, the growth condition of each strain was detected by using microplate reader. The experimental results show that, when the bacteria normally start the LuxR/I mechanism, the crp sequence has a blocking effect on the quorum sensing system.Therefore, the lack of crp sequences of bacteria produce more fluorescence. When the Plux promoter was unable to start the LuxR/I mechanism to generate fluorescence, the crp sequence was spontaneously carried out on the fluorescence expression. Control conditions, remove the effect of Plux promoter and crp sequence on the expression of fluorescence. In the case of poor nutrition, in the role of only the luxbox sequence, the Plux promoter was activated to express a trace of fluorescence. It is proved that the Plux promoter and the luxbox sequence, crp sequence influence and regulate the bacterial quorum sensing together.
Keywords: Quorum sensing; Microplate reader; Plux promoter; Crp sequence; Luxbox sequence
目录
摘要 ii
Abstract ii
目录 iv
1 绪论 1
1.1 群体感应现象 1
1.1.1 群体感应的发现 1
1.1.2 群体感应的概念 1
1.1.3 群体感应的分类 1
1.1.4 群体感应的机制 2
1.1.5 群体感应的应用和研究前景 2
2 细菌群体感应lux启动子的调控机制研究方案 4
2.1 实验材料 4
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