摘要太阳能是地球上生物生存繁衍的能源基础。光合作用对各种生物的生长和进化，甚至对人类社会都至关重要。自然界中能够进行光合作用的生物都具有各式各样的捕光天线体系，其中在绿色丝状光合细菌中存在的一种称之为绿小体的天线是已知光合生物体中最大的捕光天线构造。绿小体被膜上含有多种蛋白，是绿小体内捕获的光能传递进入光合膜的重要调控枢纽。88838

本研究采用分子生物学相关方法和技术对绿小体被膜CsmN蛋白进行了研究。主要包括以下一些内容：（1）对CsmN基因进行生物信息学分析，主要是利用NCBI网站的各种数据库和分析软件中对其全长DNA进行分析。（2）利用试剂盒提取绿色丝状细菌总基因组DNA。（3）根据生物信息分析结果来设计特异引物，以分离的总基因组为模板，PCR扩增目的基因。（4）用PCR扩增产物与sGFP（synthetic green fluorescent protein）连接，得到连接产物CsmN-GFP。（5）将CsmN-GFP与载体pEASY-Blunt-E2连接，构建重组T载体，并转化到大肠杆菌感受态细胞Trans1-T1中。（6）提取出重组质粒并转化至大肠杆菌感受态细胞BL21(DE3)中进行诱导表达。 

本研究将为绿色丝状细菌的绿小体的结构与功能的进一步研究提供理论基础。

Abstract Solar energy is the foundation of all life on the earth。 Photosynthesis is obviously important to power the growth and evolution of all kinds of life, even for human society。 The light-harvesting antenna of photosynthetic organisms exists in different types。 The so-called chlorosomes from green filamentous bacteria are the largest antenna system。 There are a variety of proteins on the envelope membrane of chlorosomes。 They play important roles as critical regulation site in energy transfer process。 

In this project，we use molecular biology methods and techniques to study CsmN protein。 The investigation includes the following experiments: (1) Conducting the bioinformatics analysis of CsmN gene, mainly including all kinds of analysis of its full length DNA by some various databases and analysis software in NCBI website。 (2) Using kit to extract the genome DNA from green filamentous bacteria。 (3) Based on the analysis of bioinformatics, designing specific primers to amplify target gene by PCR with isolation of the total genome as template。 (4) Connecting amplified product with sGFP (synthetic green fluorescent protein) to construct the connection product CsmN-GFP。 (5) Connecting CsmN-GFP with carrier pEASY-Blunt-E2 to form a recombinant plasmid and transforming the recombinant plasmid into the E。coli competent cell Trans1-T1。 (6) Extracting the recombinant plasmid and transforming the recombinant plasmid into the E。coli competent cell BL21(DE3) to make the expression of the target protein。

This study made the foundation for further research on the structure and function of chlorosomes in green filamentous bacteria。

毕业论文关键词：绿色丝状细菌； 绿小体； CsmN蛋白； 基因克隆

Keywords: green filamentous bacteria; chlorosomes; CsmN protein; gene clone

目  录

目录 3

1。引言 4

2。材料与方法 5

2。1实验材料 5

2。1。1主要实验仪器及规格 5

2。1。2主要实验试剂 5

2。1。3主要培养基及试剂的配制 6

2。2实验方法 6

2。2。1提取绿色丝状细菌总基因组DNA 6

2。2。2设计特异性引物