摘要： 多糖是生物体内重要生理活性物质。微生物多糖是微生物利用现成的碳水化合物进行二次代谢，在细胞内合成，分泌到胞外的产物，其中大多是作为荚膜和细胞外粘液的成分。近二十年来，多糖的基础研究及其开发利用取得了巨大的进展，发现了一系列具有显生物活性的多糖类成分。主要有免疫调节性、抗肿瘤、降血糖、降血脂、抗病毒等活性。微生物多糖的比植物多糖又具有生产周期短、成本低、便于实现工业化生产等优点。本课题研究酵母多糖的发酵工艺。通过单因素实验考察了培养基主要成分对发酵产多糖的影响并用正交实验确定了最优配比：结果表明优化培养基为：葡萄糖：1.50%、蛋白胨：2.00%、酵母浸膏：1.00%、 NaH2PO4：0.1%、KH2PO4：0.2%和MgSO4：0.1%。另外，还通过单因素实验确定了最适合的装液量为100mL、接种量为2.00%、培养温度为37℃、起始pH为6.5和转速为200r/m。42335

毕业论文关键字： 多糖；酵母；乙醇沉淀；硫酸-苯酚法；浊度法

Study on the preparation of Polysaccharide from a bacterial strain

Abstract: Polysaccharide is an important bioactive substance. Microbial polysaccharides are microorganisms using readily available carbohydrate for two times in the cell metabolism, synthesis, secretion of extracellular products, most of which is used as the capsule and extracellular mucus components. In the past twenty years, based on polysaccharides and its development and application has achieved great progress, found a series of bioactive polysaccharides. The main immunomodulatory, antitumor, hypolipidemic, hypoglycemic, antiviral activity. Microbial polysaccharides than plant polysaccharide and has a short production cycle, low cost, easy realization of industrial production and other advantages. The fermentation process of this research yeast polysaccharide. The main components of the medium for fermentation of Polysaccharide by orthogonal experiment to determine the optimal ratio were investigated by single factor experiments. The results showed that the optimal medium: glucose: 1.5 0%, peptone: 2.00%, yeast extract: 1.00%, NaH2PO4:0.1%, and MgSO4:0.1%. KH2PO4:0.2% also, also through the single factor experiment determined the most suitable installed fluid volume 100mL, inoculum size was 2.00% and culture temperature of 37 degrees, initial pH is 6.5 and the speed is 200r/m.

Keywords: Polysaccharide; yeast; ethanol; precipitation; sulfuric acid - phenol method; turbidity method

目录

1绪论 1

1.1多糖 1

1.1.1微生物多糖 1

1.1.2多糖的应用 2

1.1.3多糖的生物活性功能 3

1.1.4酵母及酵母多糖的功能 3

1.2多糖的研究方法 3

1.2.1多糖的提取分离 3

1.2.2多糖含量测量方法 5

2实验部分 7

2.1实验材料与仪器 7

2.1.1试剂与药品 7

2.1.2仪器 7

2.2实验方法 8

2.2.1菌种的活化 8

2.2.2细菌生长曲线的测定 8

2.2.3多糖浓度的测定 8

2.2.4菌体浓度的测量 9

2.2.5培养基单因素实验 10

2.3发酵培养基的正交法