摘要： β-苯乙醇是具有玫瑰花香的重要香料化合物，采用微生物转化生成天然β-苯乙醇是目前极具工业化价值的途径之一。本课题通过从茶树、桂花树、玫瑰花土壤样品以及酒糟样品和玫瑰花酵素样品中采集菌种，采用菌种筛选分离的方法对转化生成β-苯乙醇的微生物菌株进行筛选，并采用气相色谱分析、GC-MS分析、聚合酶链式反应等生物学手段对获取菌种进行鉴定，最终鉴定出玫瑰花土壤样品（C）、酒糟样品（E）筛选出了产β-苯乙醇的菌株，分别为菌C-2、菌E-2，桂花树（A）茶树（B）土壤样品中筛选出了产出苯乙醛的菌株。47059

毕业论文关键词： β-苯乙醇；生物转化；L-苯丙氨酸

Screening and identification of strains of β-phenylethanol production and preliminary study on conversion process

Abstract: β-phenethyl alcohol is an important Fragrance compounds having a rose scent, the use of microbial conversion generate natural β-phenethyl alcohol is one way to present highly industrialized value. This topic by collecting bacteria from tea, sweet-scented osmanthus trees, roses and soil samples and distiller's grains samples and roses enzyme samples, the method of screening of strains isolated on the conversion generates β-phenethyl l alcohol screening microbial strains, and the use of gas chromatography analysis, GC-MS analysis of biological means of polymerase chain reaction to get the bacteria were identified, and ultimately identified soil samples roses (C), distiller's grains sample (E) screened the strains producing β-phenethyl  alcohol, bacteria were C-2, strain E-2, osmanthus tree (a) tea tree (B) soil samples were screened out strains output phenylacetaldehyde.

KeyWords: β-phenylethanol；Bioconversion；L-Phenylalanine

目  录

1 绪论 1

1.1 β-苯乙醇产品概况 1

       1.1.1 β-苯乙醇的理化性质 1

1.1.2 β-苯乙醇的用途 1

1.1.3 β-苯乙醇的合成方法 2

1.2 合成β-苯乙醇的微生物 2

1.3 微生物转化法合成β-苯乙醇的代谢途径 3

1.4 国内外研究现状 4

1.4.1 国内研究现状 4

1.4.2 国外研究现状 5

1.5 选题背景及意义 6

2 实验部分 6

2.1 实验材料与仪器 6

2.2 培养基配制 7

2.2.1 培养基配置原则 7

2.2.2  产β-苯乙醇菌种筛选的培养基 7

2.2.3 产β-苯乙醇菌种的转化培养基 9

2.3 实验方法 9

2.3.1 菌种的初步筛选 9

2.3.2 菌种的生物转化实验 10

2.3.3 产物分析和菌种鉴定 10

3、结果与讨论 13

3.1 筛菌结果 13

3.2 生物转化实验 14

3.2.1 气相分析 14

3.2.2 GC-MS分析 16

3.3 PCR与凝胶电泳