摘要 目的：以C57BL/6J小鼠为动物模型，研究红牛饮料对小鼠运动耐力的影响。方法：选取12周龄健康雌性小鼠12只，首先进行适应性喂养1周，之后以每组体重相似性为标准分为2组，一组为对照组，一组为处理组。喂养处理4周后，对小鼠进行一次性负重竭力游泳实验（其负重物为小鼠体重3%），建立小鼠疲劳模型并记录竭力游泳时间。竭力游泳实验结束后处死小鼠，迅速分离血清、部分肝脏及其腿部肌肉组织，对上述组织分别测定血尿素氮（BUN），丙二醛（MDA），肝糖原以及肌糖原含量。结果：相比对照组，处理组的竭力游泳时间显著延长，耐力提高；血清MDA含量显著减少，抗氧化能力增强；而血清BUN含量变化不大，说明小鼠非蛋白质能量代谢能力并未得到显著增强。结论：一定量的红牛饮料能够在某种程度上提高小鼠的运动耐力。48871

毕业论文关键词：红牛；小鼠；运动耐力；抗疲劳；代谢能力

Effect of Red Bull sports drink on endurance of mice

Abstract Purpose: C57BL / 6 j mice as animal model, study the effect of red bull to exercise endurance in mice. Methods: select 12 only 12 weeks of healthy female mice, first of all to adaptive feeding 1 week, after based on weight similarity in each group was pided into two groups, one group for the control group, a group for processing. Feed processing after 4 weeks, one-time weight to swimming experiments in mice (the load for mice weight 3%), fatigue in mice model was established and recorded to swimming time. Trying to swim after the experiment put to death in mice, the rapid separation of serum, liver and leg muscle tissue, the above organizations, respectively, blood urea nitrogen (BUN), malondialdehyde (MDA), liver glycogen and muscle glycogen content. Results: compared with control group, treatment group trying to swim) were significantly prolonged, endurance; Significantly reduce serum MDA content, antioxidant ability enhancement; And serum BUN content changed little, explain the protein has not been significantly enhance its capability of energy metabolism in mice. Conclusion: a certain amount of red bull can enhance the endurance of the movement of mice to a certain extent. 

Key words: Red bull; Mice; Movement endurance; Fatigue resistance; Ability to metabolize 

目    录

摘  要 1

Abstract 1

引言 2

1材料与方法 2

1.1材料与试剂 2

1.2仪器与设备 3

1.3小鼠处理与指标测定 3

1.3.1小鼠饲养与分组 3

1.3.2小鼠采食量与体重测定 3

1.3.3小鼠竭力游泳时间测定 3

1.3.4小鼠组织与血清的分离与提取 4

1.3.5血尿素氮BUN含量测定 4

1.3.6丙二醛MDA含量测定 4

1.3.7肝糖原与肌糖原含量测定 5

1.4数据分析 6

2结果与分析 6

2.1红牛对小鼠体重的影响 6

2.2红牛对小鼠竭力游泳时间的影响 7

2.3红牛对小鼠体内血尿素氮含量的影响 8

2.4红牛对小鼠体内丙二醛含量的影响 9

2.5红牛对小鼠体内肝糖原和肌糖原含量的影响