摘要: 目的: 探究乐虎保健饮料的抗疲劳作用。方法: 准备12只健康雄性小鼠，平均分成2组，处理组和对照组。然后对处理组小鼠饮水中每天加入一定量的乐虎保健饮料，对照组小鼠不做处理。喂食期间，同时对处理组和对照组称量体重及采食量等指标。4周后，对实验组和对照组的小鼠进行一次性负重力竭游泳，测游泳时间，然后对小鼠进行解剖，收集血液样品，分离肝脏和肌肉组织，分别测血尿素氮（BUN）、丙二醛（MDA）、肝糖原和肌糖原含量。结果：相比对照组，乐虎保健饮料能显著延长小鼠的力竭游泳时间，降低血清MDA增量，小鼠肝糖原储备。结论: 饮用一定量的乐虎保健饮料能够提高小鼠的抗疲劳能力。48872

毕业论文关键词: 小鼠；乐虎保健品；抗疲劳；抗氧化；丙二醛

Study on the anti-fatigue effect of Hi-tiger on mice

Abstract:To investigate anti-fatigue effect of Hi-tiger on mice, 12 male mice were pided into control and GBE-treated (64.8mg/day/kg) after one week adaptive treatment. After four-weeks of treatment, one exhaustion swimming test was performed for all mice and the swimming times were recorded. Then, all mice were sacrificed and the blood, liver and muscle tissues were sampled to measure the content of blood urea nitrogen (BUN), malondialdehyde (MDA), liver glycogen and muscle glycogen, respectively. The results showed that, compared with the control group, the swimming time of EGB-treated group significantly prolonged, the content of malondialdehyde (MDA) decreased and the content of liver glycogen increased significantly. In conclusion, some dose of EGB supplements can improve effectively the anti-fatigue effect on mice.

 Key words: mice; hi-tiger; anti-fatigue; antioxidant; MDA

目    录

摘  要 1

Abstract 1

引言 2

1材料与方法 2

1.1材料与试剂 2

   1.1.1实验动物 2

   1.1.2实验药品 2

1.2仪器与设备 3

1.3小鼠处理与指标测定 3

1.3.1小鼠喂养及分组 3

1.3.2小鼠血清和组织的分离与提取 3

1.3.3小鼠体重测定 3

1.3.4小鼠竭力游泳时间测定 3

1.3.5 血尿素氮BUN含量测定 4

1.3.6丙二醛MDA含量测定 4

1.3.7肝糖原含量测定 5

1.3.8肌糖原含量测定 5

1.4数据分析 6

2结果与分析 6

2.1乐虎保健饮料对小鼠体重的影响 6

2.2乐虎保健饮料对小鼠竭力游泳时间的影响 7

2.3乐虎保健饮料对小鼠体内血尿素氮含量的影响 8

2.4乐虎保健饮料对小鼠体内丙二醛含量的影响 9

2.5乐虎保健饮料对小鼠体内肝糖原含量的影响 9

2.6乐虎保健饮料对小鼠体内肌糖原含量的影响 10

3讨论 10

参考文献