摘要唐古特白刺是一种药食同源植物，在民间用药历史悠久，其果实营养丰富，富含黄酮，多酚类等化合物，有许多生物学活性，具有广阔的应用前景。对唐古特白刺果实成分的分离纯化，为白刺的进一步药用开发提供理论依据。本次实验用95%的乙醇提取唐古特白刺果实中的成分后，分别用二氯甲烷，乙酸乙酯，正丁醇，甲醇萃取分段。最后从二氯甲烷段经多次柱层析分离得到一化合物，经谱图解析为β-谷甾醇。β-谷甾醇因其具有多种生物学活性及其独特的结构，广泛地应用于临床和工业上。64757

毕业论文关键词  唐古特白刺  生物学活性  β-谷甾醇

毕业设计说明书（论文）外文摘要

Title  The chemical ingredients in Nitraria tangutorum Bobr.

Abstract

Nitraria tangutorum Bobr is one traditional Chinese medicine. Its fruit is rich with nutrients, flavonoids, polyphenols, and other compounds, which shows lots of bio-activities. To provide its basic evidences for the further development of the medical application of N. tangutorum., The constituents in N. tangutorum.are isolated. In this experiment, firstly, the ingredients of N. tangutorum were extracted with 95% ethanol; secondly, it is partitioned with dichloromethane, ethyl acetate, n-butanol, methanol respectively. After several column chromatography separations, a compound named β-sitosterol was isolated from the dichloromethane segment eventually and characteried by MS and NRM spectroscopy. With a variety of bio-activities and its unique structure, β-sitosterol would be widely applied to clinic and industry.

Keywords  Nitraria tangutorum Bobr  bio-activity  β-sitosterol   

                       目录

1  绪论 1

1．1   白刺的生物学特征 1

1.1.1  白刺的分布 1

1.1.2  生态特征 2

1.2 白刺成分研究 3

1.2.2 白刺的化学成分 3

1.2.3白刺果实色素 5

1.3 白刺的药理活性 5

1.3.1 心血管系统活性 5

1.3.2 抗菌及抗病毒活性 6

1.3.3抗肿瘤活性 6

1.3.4 抗氧化自由基活性 6

1.3.5 抗炎，镇痛活性 6

1.4 白刺化合物的提取与分离 7

1.4.1 实验提取分离方法 7

1.4.2  唐古特白刺种子油的分离 7

1.5白刺化合物的检测 7

1.5.1 黄酮类的检测 7

1.5.2 白刺中花色苷的检测 9

2. 实验材料与方法 10

2.1实验材料 10

2.2 实验试剂与仪器 10

2.3　实验方法 10

2.3.1 唐古特白刺的粗提 11

2.3.2 二氯甲烷部位分离纯化 11

3. 实验结果与讨论