摘  要：土壤酶在土壤能量转化和物质循环中具有重要作用，是土壤肥力的重要指标。土壤转化酶对土壤中碳循环有重要作用, 可以反映土壤熟化水平，是研究最多的土壤酶之一。江苏省淮阴地区为重要的农业产区，盛产多种农产品。本文采用3,5-二硝基水杨酸比色法，对淮阴地区多种农田土壤转化酶开展了初步的调查研究，对了解该地区土壤特性和科学合理施肥具有一定的指导意义。调查研究结果表明：淮阴地区不同田区的土壤转化酶含量不同。同一作物相同土壤不同土层的转化酶活性也不同，随着土层剖面加深，土壤转化酶的活性降低。68381

毕业论文关键字：淮阴地区，土壤酶，土壤转化酶活性

Abstract: Soil enzymes play an important role in energy conversion in the soil and material cycle and that is one of the important indicators of soil fertility. Soil invertase is significant to soil carbon cycle. And it can reflect the soil maturation level, which is one of the most popular soil enzymes. As the important agricultural regions of Jiangsu, Huaiyin district is rich in a variety of agricultural products. According to the colorimetry of 3, 5 two nitro salicylic acid, preliminary investigations and studies of soil catalase were carried out in various huaiyin regions, which has a certain guiding significance to know the soil characteristics of the region and the scientific and reasonable fertilization methods. As is shown by the research results, soils from various Huaiyin districts contain different contents of soil invertase. Moreover, even from the same crop and soil , the activity of invertase in different soil layers is distinct as well. The deeper soil layers profile, the lower activity of soil invertase will be.

Key words: Huaiyin district，soil enzyme，activity of soil invertase

目录

1  前言 4

1.1  土样区域概况 4

2  材料与方法 4

2.1  试剂及相关溶液 4

2.2  土壤样品的采集与处理 5

2.3  土壤含水量测定 5

2.4  PH值的测定 5

2.5  土壤转化酶活性测定 5

2.5.1  催化时间对土壤转化酶测定结果的影响 5

2.5.2  不同作物农田土壤转化酶活性测定 6

3  测定结果 6

3.1  土壤含水量结果 6

3.2  土壤pH值 6

3.3  催化时间对土壤转化酶测定结果 7

3.4  不同作物农田土壤转化酶活性测定结果 7

3.5  葡萄糖标准曲线 7

4  结果与分析 8

4.1  计算结果 8

4.2  分析 8

4.2.1  规律 8

4.2.2  原因 8

结论 10

参考文献 11

致谢 12

1 前言

我国从古至今都是一个农业大国，农业用地数量多，但人均占有量少。宜林地较多，宜农地较少。后备土地资源不足。在化肥应用于农业生产之前，我国农业生产主要靠农家肥和扩大耕地面积来促进作物增产。而化肥问世后，由于养肥含量高，便捷等因素，致使其过量使用，导致局部地区土壤板结、有机质不足、保水性和透气性下降。农业生产正面临一系列危机。因此了解土壤肥力，知晓土壤中循环和能量转化对于日后的农业生产有着重要的意义。论文网