萝卜S受体蛋白激酶同源建模、评价和通路分析
关键词：S受体蛋白激酶；自交不亲和；同源建模；生物信息学
Homology modeling,evaluation and pathway analysis of S receptor kinase in Raphanus sativus L
Abstract: To understand the structure and function of radish S receptor protein kinases, its amino acid composition, terms of physicochemical properties, subcellular localization, signal peptide, transmembrane domain and secondary structure were predicted by bioinformatics ways, then its tertiary structure was obtained by homology modeling, and the modeled structure qulity was estimated using Laplace scattergram; the protein active site of information was predicted. The results showed, S protein kinase receptor consists of 853 amino acids; presence of a transmembrane region at its N- terminal amino acids 7-29, the direction from the inside outward a,446 -465 amino acid transmembrane region is an outside, the protein is localized in the cytoplasm of a secreted protein, comprising a plurality of different phosphorylation sites; two structures to expand the beam ( 25.09 %) and random coil ( representing 47.48% ) there are mainly 14 unstable region, four potential globular protein region; tertiary structure of the α- helical segments 14 and three groups β- folded configuration, wherein a set of parallel β- pleated sheet structure of the folded single-chain β 5 , is located inside the protein the other two β- pleated sheet structure consists of several smaller single β chains folded , near the external protein is an α + β -like protein ; the protein has seven different mold , or may be involved in biochemical reactions performed in different different functions; the protein with cabbage , rape seed S is equivalent to the receptor protein kinases high similarity , evolutionary distance closer . These results further theoretical basis.
Keywords: S receptor protein kinase; Self-incompatibility; homology modeling; Bioinformatics
目 录
摘要 1
引言 1
1 材料和方法 3
1.1 种子序列及其来源 3
1.2 生物信息学分析软件 3
2 结果与分析 3
2.1 S受体蛋白激酶序列分析 3
2.2 S受体蛋白激酶理化性质分析 4
2.3 S受体蛋白激酶磷酸化预测 5
2.4 S受体蛋白激酶亚细胞定位、跨膜区和信号肽分析 5
2.5 S受体蛋白激酶二级结构、蛋白紊乱区、球蛋白区预测 6
上一篇：氧化应激下酵母蛋白的表达模式分析